Evidence map›Paper›PMID 40575978›Full record

ArticleAnimal bioscience2025

Effects of the DUSP6 gene on the proliferation and differentiation of porcine subcutaneous preadipocytes.

Xiangxiang Yang, Xiaohan Sun, Zimeng Du, Jundong Yi, Qiuyan Wang, Ding Yin, Yalan Zhang, Xiaoling Ding, Xianrui Zheng, Xiaodong Zhang and 2 more

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In one paragraph

Article in Animal bioscience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Xiangxiang YangCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Xiaohan SunCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Zimeng DuCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Jundong YiCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Qiuyan WangCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Ding YinCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Yalan ZhangCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Xiaoling DingCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Xianrui ZhengCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Xiaodong ZhangCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Zongjun YinCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Yueyun DingCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.

Funding

Anhui Provincial Modern Industrial Technology System of Swine AHCYJSTX-05-01Anhui Provincial Modern Industrial Technology System of Swine AHCYJSTX-05-03Collaborative Innovation Project of Universities in Anhui Province GXXT-2023-059Joint Research Project on Local Pig Breeding in Anhui Province 340000211260001000431National Key Research and Development Program of China 2021YFD1301200National Natural Science Foundation of China 32573167
6 · The paper itself

Abstract

objectiveDual-specificity protein phosphatase 6 (DUSP6), also known as mitogenactivated protein kinase phosphatase 3 (MKP-3), was considered as a functional candidate gene for white fat accumulation in mice. However, the physiological function of the DUSP6 gene on white adipocyte adipogenesis in farm animals remains unknown. In this study, we aimed to clarify the effect of DUSP6 on porcine subcutaneous preadipocyte proliferation and differentiation.

methodsWe first make clear that the patterns of DUSP6 expression is associated with fat contents in porcine fat deposition related tissues. Porcine subcutaneous preadipocytes were isolated and induced to differentiation. Small interfering RNAs were applied to deplete DUSP6. MTT assay, CCK-8 analysis, Oil Red O staining, triglyceride determination and reverse transcription quantitative polymerase chain reaction were applied to study the regulatory role of DUSP6 during adipocyte adipogenesis in pigs.

resultsWe found that the expression levels of DUSP6 were significantly higher in backfat and longissimus dorsi tissues from fat-type pigs than in those from lean-type pigs. Consistently, the significantly induced expression of DUSP6 was also observed in differentiated adipocytes. In addition, knockdown of DUSP6 greatly inhibited preadipocytes proliferation, through the decreased cell viability and downregulated mRNA expressions of cell proliferation-associated genes, including PCNA, CDK1, CDK2. Furthermore, knockdown of DUSP6 significantly inhibited preadipocytes differentiation, as evidenced by markedly reduced lipid droplet formation, attenuated triglyceride accumulation and downregulated expression levels of adipogenic transcription masters (PPARγ, C/EBPβ, FASN and FABP4) in DUSP6 knockdown cells.

conclusionOur results demonstrate that DUSP6 is required for white adipocyte adipogenesis in pigs.

Indexed as

Cell Proliferation and DifferentiationDUSP6PigPreadipocytes

Identifiers

PMID40575978
PMCPMC12580764

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.