ArticleAnimal bioscience2025
Transcriptional regulation of porcine PABPN1 gene in adipogenesis.
Article in Animal bioscience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Article
- Beyond the Poly(A) Tail: The Expanding Functional Landscape of PABPN1 and Its Dysregulation in OPMD and Cancer.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
objectiveThis study aims to reveal the transcriptional regulatory mechanism and effects of poly(A)-binding protein nuclear 1 (PABPN1) on adipogenesis, along with associated polymorphisms.
methodsTranscription factors were identified using dual-luciferase reporter assay, overexpression techniques site-directed mutagenesis, real-time quantitative polymerase chain reaction (qPCR), electrophoretic mobility shift assays and chromatin immunoprecipitation-qPCR. Preadipocyte differentiation was measured with gain- and loss-of-function, Oil Red O staining and extraction assays. Single nucleotide polymorphisms (SNPs) were identified with direct sequencing of PCR products in the promoter, and the effects of these SNPs on PABPN1 expression were identified with dual-luciferase reporter assay.
resultsCCAAT/enhancer-binding protein (C/EBP) α and β regulate PABPN1 expression by directly binding to its promoter. PABPN1 promotes the preadipocyte differentiation in pigs. Three SNPs were identified, with the Haplotype GCC mutation significantly increasing the promoter activity of PABPN1.
conclusionPABPN1 promotes the preadipocyte differentiation as a downstream gene of C/EBP α and β. Haplotype GCC may serve as a molecular marker for selection of fat traits in pigs.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.