Evidence map›Paper›PMID 40576446›Full record

ArticleMolecular microbiology2025

Cyclic Di-AMP Affects Cell Membrane Integrity of Streptococcus pneumoniae.

Tiffany M Zarrella, Jianle Gao, Nathan Forrest, Elijah Crosbourne, Kaibo Cui, Guangchun Bai

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Tiffany M ZarrellaDepartment of Immunology and Microbial Disease, Albany Medical College, Albany, New York, USA.
Jianle GaoDepartment of Immunology and Microbial Disease, Albany Medical College, Albany, New York, USA.
Nathan ForrestDepartment of Immunology and Microbial Disease, Albany Medical College, Albany, New York, USA.
Elijah CrosbourneDepartment of Immunology and Microbial Disease, Albany Medical College, Albany, New York, USA.
Kaibo CuiDepartment of Immunology and Microbial Disease, Albany Medical College, Albany, New York, USA.
Guangchun BaiDepartment of Immunology and Microbial Disease, Albany Medical College, Albany, New York, USA.

Funding

Pneumonia BiologyR35HL135756 · NHLBI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI MIZGERD, JOSEPH P · 2017 to 2023
$6.6M
Albany Medical CollegeNHLBI NIH HHS R35 HL135756NIH HHS R35HL135756
6 · The paper itself

Abstract

Competence is an important bioprocess for Streptococcus pneumoniae. Previously, we demonstrated that the bacterial second messenger cyclic di-adenosine monophosphate (c-di-AMP) modulates pneumococcal competence. Surprisingly, cdaA*, a strain producing less c-di-AMP due to a point mutation in the diadenylate cyclase CdaA, is susceptible to competence-stimulating peptide (CSP). In this study, we screened cdaA* suppressor mutants resistant to CSP to explore the underlying mechanism. Of 14 clones sequenced, nine clones possessed mutations in the c-di-AMP phosphodiesterase Pde1, indicating that the susceptibility to CSP of the cdaA* strain is correlated to c-di-AMP levels. Another two clones exhibited a mutation in FabT, a transcription factor controlling cell membrane fatty acid biosynthesis. We further showed that deletion of fabT, disruption of the FabT-binding site within the P

Indexed as

Cell MembraneDinucleoside PhosphatesStreptococcus pneumoniaeBacterial ProteinsCyclic AMPGene Expression Regulation, BacterialMutationPhosphoric Diester HydrolasesPromoter Regions, GeneticTranscription FactorsBacterial ProteinsCyclic AMPcyclic diadenosine phosphateDinucleoside PhosphatesPhosphoric Diester HydrolasesTranscription FactorsCdaAc‐di‐AMPcompetencediadenylate cyclasemembrane integritypotassium transportStreptococcus pneumoniae

Identifiers

PMID40576446
PMCPMC12354235

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.