ArticleMetabolomics : Official journal of the Metabolomic Society2025
Metabolomic alterations in human brain microvascular endothelial cells induced by traumatic injury.
Article in Metabolomics : Official journal of the Metabolomic Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Mass Spectrometry-Based Metabolomics in Pediatric Health and Disease.Metabolites · 2026Review
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6 authors.
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Abstract
introductionAltered metabolic pathways are critical in the progression of traumatic brain injury (TBI). Identifying differentially abundant metabolites (DAMs) from specific cell types can offer valuable diagnostic and prognostic insights.
objectiveThis study aimed to characterize the metabolomic profile of injured human brain microvascular endothelial cells (hBMEC) at 2-, 12-, 24-, and 48 h post-injury.
methodsUsing an in vitro TBI model, we analyzed metabolites in cell culture media through a combination of direct injection mass spectrometry and a custom reverse-phase LC-MS/MS assay. We evaluated 644 metabolites at each time point.
resultsPhosphatidylcholines were significantly upregulated across all time intervals. At 2- and 12 h post-injury, the most significantly upregulated metabolites included sphingomyelin (OH) C22:1, ethylmalonic acid, and methylhistidine, while guanosine and the combination of butyric acid + isobutyric acid were the most downregulated. At 24 and 48 h, deoxyadenosine and inosine, respectively, emerged as the most upregulated metabolites, with butyric acid + isobutyric acid and quinoline-4-carboxylic acid showing the greatest downregulation.
conclusionMetabolomic profiling identified various DAMs after traumatic injury that are linked to human endothelial dysfunction. Future experiments should expand the number of metabolites measured to determine the underlying signaling pathways.
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