Evidence map›Paper›PMID 40577132›Full record

ArticleCell reports2025

RAP proteins regulate apicoplast noncoding RNA processing in Plasmodium falciparum.

Thomas Hollin, Zeinab Chahine, Steven Abel, Charles Banks, Charisse Flerida A Pasaje, Todd Lenz, Jacques Prudhomme, Caitlyn Marie Ybanez, Anahita S Abbaszadeh, Jacquin C Niles and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Thomas HollinDepartment of Molecular, Cell and Systems Biology, University of California, Riverside, Riverside, CA, USA.
Zeinab ChahineDepartment of Molecular, Cell and Systems Biology, University of California, Riverside, Riverside, CA, USA.
Steven AbelDepartment of Molecular, Cell and Systems Biology, University of California, Riverside, Riverside, CA, USA.
Charles BanksStowers Institute for Medical Research, Kansas City, MO, USA.
Charisse Flerida A PasajeDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Todd LenzDepartment of Molecular, Cell and Systems Biology, University of California, Riverside, Riverside, CA, USA.
Jacques PrudhommeDepartment of Molecular, Cell and Systems Biology, University of California, Riverside, Riverside, CA, USA.
Caitlyn Marie YbanezDepartment of Molecular, Cell and Systems Biology, University of California, Riverside, Riverside, CA, USA.
Anahita S AbbaszadehDepartment of Molecular, Cell and Systems Biology, University of California, Riverside, Riverside, CA, USA.
Jacquin C NilesDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Laurence FlorensStowers Institute for Medical Research, Kansas City, MO, USA.
Karine G Le RochDepartment of Molecular, Cell and Systems Biology, University of California, Riverside, Riverside, CA, USA. Electronic address: karine.leroch@ucr.edu.

Funding

RAPs-mediated post-transcriptional control in Apicomplexan parasitesR01AI142743 · NIAID · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI LE ROCH, KARINE GAELLE · 2018 to 2022
$2.8M
Illumina NovaSeq 6000 Sequencing SystemS10OD026929 · OD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI JEPSEN, KRISTEN LYNN · 2019 to 2019
$600k
RNA-Binding Proteins and RNA-Dependent Proteins - An Emerging Role for RNAs in Plasmodium BiologyR21AI191405 · NIAID · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI Laurence Alexandra Florens, Karine Gaelle Le Roch · 2025 to 2026
$399k
NIAID NIH HHS R01 AI142743NIAID NIH HHS R21 AI191405NIH HHS S10 OD026929
6 · The paper itself

Abstract

The human malaria parasite, Plasmodium falciparum, contains a non-photosynthetic and essential plastid called the apicoplast. This organelle is of major interest for its unique biology and potential as an attractive drug target. In this study, we characterize PfRAP03 and PfRAP08, two members of the RAP (RNA-binding domain abundant in apicomplexans) protein family. We generate inducible knockdown lines in P. falciparum to validate that both RAP proteins are essential for parasite survival and localize to the apicoplast. Transcriptomic analysis demonstrates that PfRAP03 and PfRAP08 depletion significantly affect apicoplast gene expression. Using enhanced crosslinking immunoprecipitation sequencing (eCLIP-seq) method, we show that apicoplast ribosomal RNAs and transfer RNAs are the targets of PfRAP03 and PfRAP08, respectively. Collectively, our results establish the role of these RAP proteins in controlling apicoplast gene expression in P. falciparum, revealing parasite-specific organellar pathways with biomedical significance.

Indexed as

ApicoplastsPlasmodium falciparumProtozoan ProteinsRNA-Binding ProteinsRNA Processing, Post-TranscriptionalRNA, UntranslatedHumansProtozoan ProteinsRNA-Binding ProteinsRNA, UntranslatedapicoplastCP: MicrobiologymalariaPlasmodiumpost-transcriptional regulationRAP proteinRNA binding

Identifiers

PMID40577132
PMCPMC12370240

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.