Evidence map›Paper›PMID 40580291›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Haplotype and Presence-Absence Variation Analysis Using High-Resolution Melting (HRM) Analysis for Genotyping Genes of Interest.

Anthony Torres, Robert Givens, Reginald Gaudino

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Anthony TorresCannabis Research Institute, Discovery Partners Institute, University of Illinois System, Chicago, IL, USA.ORCID https://orcid.org/0000-0003-3631-3079
Robert GivensFront Range Biosciences, Lafayette, CO, USA.
Reginald GaudinoCannabis Research Institute, Discovery Partners Institute, University of Illinois System, Chicago, IL, USA.ORCID https://orcid.org/0000-0001-5902-5606

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter outlines a basic protocol for DNA extraction, applicable to both FTA cards and leaf pieces. The extracted DNA then undergoes a pre-amplification enrichment reaction, followed by dilution and preparation for input into a real-time amplification with High-Resolution Melt (HRM) detection. The resulting melting genotypes are produced for comparison and analysis, providing a practical approach for genotyping studies. The flexibility of the method allows specific analytical output and high throughput analysis of a given DNA sequence that possesses inherent variability between individuals. This protocol describes the crude approach of extracting DNA from samples, preparing a real-time HRM reaction, and analyzing the result.

Indexed as

Genotyping TechniquesHaplotypesDNA, PlantGenotypeNucleic Acid DenaturationReal-Time Polymerase Chain ReactionTransition TemperatureDNA, PlantAlleleAmpliconDNADNA extractionFTA cardsGenotypingHRMMelting differenceMelting tempPre-amplificationPrimersProbeqPCRReal-time HRMStructural and allelic variationsTm

Identifiers

PMID40580291

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.