Evidence map›Paper›PMID 40586279›Full record

ArticleRegenerative medicine2025

Coagulation activation and hepatic engraftment of human amnion-derived cells after intraportal transplantation in rats.

Miyako Tanaka, Kazuaki Tokodai, Kaoru Okada, Masato Sato, Hitomi Okita, Takako Ito, Tetsuro Hoshiai, Masatoshi Saito, Toshio Miki, Michiaki Unno and 2 more

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Article in Regenerative medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Miyako TanakaDepartment of Surgery, Tohoku University, Sendai, Japan.ORCID 0000-0002-0607-8250
Kazuaki TokodaiDepartment of Surgery, Tohoku University, Sendai, Japan.
Kaoru OkadaDepartment of Surgery, Tohoku University, Sendai, Japan.
Masato SatoDepartment of Surgery, Tohoku University, Sendai, Japan.
Hitomi OkitaTransplantation and Regenerative Medicine Center, Tohoku University Hospital, Sendai, Japan.
Takako ItoTransplantation and Regenerative Medicine Center, Tohoku University Hospital, Sendai, Japan.
Tetsuro HoshiaiDepartment of Obstetrics and Gynecology, Tohoku University, Sendai, Japan.
Masatoshi SaitoDepartment of Maternal and Fetal Therapeutics, Tohoku University, Sendai, Japan.
Toshio MikiDepartment of Physiology, Nihon University, Itabashi-Ku, Japan.
Michiaki UnnoDepartment of Surgery, Tohoku University, Sendai, Japan.
Takashi KameiDepartment of Surgery, Tohoku University, Sendai, Japan.
Masafumi GotoDivision of Transplantation and Regenerative Medicine, Tohoku University, Sendai, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimsHuman amniotic epithelial cells (hAECs) have emerged as a promising cell source for regenerative medicine, with intraportal transplantation as a potential delivery route. However, the extent to which hAECs induce an immediate inflammatory response remains unclear. This study investigated tissue factor (TF) expression in isolated hAECs and assessed coagulation activation following intraportal transplantation in a rat model. MATERIALS AND

methodsTF expression was analyzed before and after cryopreservation, while thrombin - antithrombin (TAT) complex levels were measured to evaluate coagulation activation. To assess engraftment and hepatocyte-like function, hAECs were transplanted into non-albuminemic rats, followed by serial measurements of serum human albumin levels and histological liver analysis.

resultsThe findings indicate that hAECs express TF pre- and post-cryopreservation. Intraportal transplantation resulted in a significant increase in plasma TAT levels, which was mitigated by heparin administration. Furthermore, human albumin levels increased post-transplantation, and albumin-positive cells were detected in the liver on day 21.

conclusionThese results suggest that intraportal hAEC transplantation is a feasible approach for hepatic engraftment; however, TF expression may trigger coagulation activation, potentially leading to an instant blood-mediated inflammatory reaction. Further research is warranted to optimize anticoagulation strategies and evaluate long-term engraftment efficacy for clinical applications.

Indexed as

AmnionBlood CoagulationEpithelial CellsLiverAnimalsCryopreservationFemaleHumansMalePortal VeinRatsRats, Sprague-DawleyThromboplastinThromboplastinamniotic/placental stem cellsCoagulation activationinstant blood-mediated inflammatory reactionintraportal administrationtissue factor

Identifiers

PMID40586279
PMCPMC12269652

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.