ArticleNucleic acids research2025
The quantitative impact of 3'UTRs on gene expression.
Article in Nucleic acids research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Large serine recombinase-mediated gene insertion for high-throughput screens: advantages, design principles, and applications.Nucleic acids research · 2026Review
- Characterizing highly conserved fragments in 3'UTRs via statistical and transfer learning approaches.NAR genomics and bioinformatics · 2026Article
- Platform-dependent performance of P2A and IRES linkers in multicistronic gene expression.Genes & genomics · 2026Article
- A protocol for computational design of mRNA vaccines with high functionality and specificity.Biology direct · 2026Article
- PTBP1 controls miRNA loading on target RNAs: lessons from the CyCoNP lncRNA.RNA (New York, N.Y.) · 2026Article
- Genetic Association and Functional Prediction of PTEN and TSC1 3'UTR Variants in Autism Spectrum Disorder Among Tunisian Patients.Molecular neurobiology · 2025Article
- Spatiotemporal dynamics and selectivity of mRNA translation during mouse pre-implantation development.Nucleic acids research · 2025Article
- Transcriptome-wide prediction of heat-sensitive RNA structures inFrontiers in plant science · 2025Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Control of gene expression is fundamental to biology, and post-transcriptional regulation is an important component of this process. In mammals, the 3'UTR in particular serves as a major source of regulatory information within the transcript. Here we developed an accurate massively parallel reporter assay (MPRA) system to evaluate the impact of >1400 full-length human 3'UTRs on RNA abundance, stability, translational regulation, and total protein output. We demonstrated that our MPRA is consistent with regulation of the corresponding endogenous transcripts. We used the MPRA datasets to model the relative contributions of RNA abundance and translational efficiency toward total 3'UTR-mediated regulation, revealing an unexpectedly large role for 3'UTR-specified translational control, and providing additional evidence that much of 3'UTR-encoded regulation is mediated by concerted regulation of translation plus decay. We observed relationships between GC content and 3'UTR length and different modes of regulation, and identified sequence motifs corresponding to regulatory RNA-binding proteins associated with mediating 3'UTR-dependent gene expression. We compared regulation from >1400 3'UTRs under control of two dissimilar promoters, which revealed promoter-associated differences in post-transcriptional regulation for certain 3'UTRs. Together, this dataset represents a comprehensive characterization of 3'UTR-mediated quantitative regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.