Evidence map›Paper›PMID 40587001›Full record

ArticleAnatomical science international2025

Visualization of nanostructures in neuronal growth cones and axons using super-resolution structured illumination microscopy.

Motohiro Nozumi, Michihiro Igarashi

Abstract read
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In one paragraph

Article in Anatomical science international, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Motohiro NozumiDepartment of Molecular Cell Biology, School of Medicine, and Graduate School of Medical and Dental Sciences, Niigata University, 1-757 Asahimachi-Dori, Chuo-Ku, Niigata, 951-8510, Japan. mnozumi@med.niigata-u.ac.jp.ORCID http://orcid.org/0000-0001-5972-2719
Michihiro IgarashiDepartment of Molecular Cell Biology, School of Medicine, and Graduate School of Medical and Dental Sciences, Niigata University, 1-757 Asahimachi-Dori, Chuo-Ku, Niigata, 951-8510, Japan.

Funding

Japan Agency for Medical Research and Development #19gm1210007s0101Japan Agency for Medical Research and Development #20gm1210007s0102Japan Agency for Medical Research and Development #21gm1210007s0103Japan Agency for Medical Research and Development #22gm1210007s0104Japan Agency for Medical Research and Development #23gm1210007s0105Japan Agency for Medical Research and Development #24gm1210007s0106Japanese Foundation for Research and Promotion of Endoscopy #18H04013Japanese Foundation for Research and Promotion of Endoscopy #18H04670Japanese Foundation for Research and Promotion of Endoscopy #20H03597Japan Society for the Promotion of Science #17KK0144Japan Society for the Promotion of Science #18K06459Japan Society for the Promotion of Science #21K19305
6 · The paper itself

Abstract

In neurons, which possess highly organized cellular structures, such as growth cones, axons, synapses, and dendritic spines, precise determination of molecular localization is a powerful approach for elucidating cellular functions. Super-resolution microscopy has revealed higher-order structures and molecular distributions that were previously undetectable using conventional confocal microscopy. Among super-resolution techniques, structured illumination microscopy (SIM) is particularly well-suited for live-cell imaging. In this study, we present a detailed methodology for observing growth cones and axons using SIM. Our SIM imaging of primary mouse neurons revealed that the phosphorylated GAP-43 localizes within axons in a pattern consistent with the membrane-associated periodic skeleton.

Indexed as

AxonsGrowth ConesMicroscopyNanostructuresNeuronsAnimalsCells, CulturedGAP-43 ProteinMiceGAP-43 ProteinAxonGAP-43Growth coneMembrane-associated periodic skeletonStructured illumination microscopy (SIM)

Identifiers

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.