Evidence map›Paper›PMID 40593413›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Mapping Antibody Epitopes by Solution NMR Spectroscopy: Practical Considerations.

Elia Tamagnini, Luca Simonelli, Mattia Pedotti, Luca Varani

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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Elia TamagniniInstitute for Research in Biomedicine, Università della Svizzera italiana (USI), Bellinzona, Switzerland.
Luca SimonelliInstitute for Research in Biomedicine, Università della Svizzera italiana (USI), Bellinzona, Switzerland.
Mattia PedottiInstitute for Research in Biomedicine, Università della Svizzera italiana (USI), Bellinzona, Switzerland.
Luca VaraniInstitute for Research in Biomedicine, Università della Svizzera italiana (USI), Bellinzona, Switzerland. luca.varani@irb.usi.ch.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Identifying an epitope, the region of the antigen in contact with an antibody, is useful in both basic and pharmaceutical research, as well as in vaccine design. Solution NMR spectroscopy is particularly well suited to residue-level characterization of intermolecular interfaces, including antibody-antigen interactions, and thus to epitope identification. Here we describe the use of NMR for the characterization of protein epitopes, focusing on experimental protocols and practical considerations, highlighting the advantages and drawbacks of the approach.

Indexed as

AntibodiesEpitope MappingEpitopesNuclear Magnetic Resonance, BiomolecularHumansMagnetic Resonance SpectroscopyAntibodiesEpitopesAllosteric effectsAntibodyAntigenChemical shift mappingChemical shift perturbationEpitope mappingSolution NMR

Identifiers

PMID40593413

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.