Evidence map›Paper›PMID 40593989›Full record

ArticleScientific reports2025

Identification of aging-related biomarkers and immune infiltration analysis in renal stones by integrated bioinformatics analysis.

Yuanzhao Wang, Nana Chen, Bangqiu Zhang, Pingping Zhuang, Bingtao Tan, Changlong Cai, Niancai He, Hao Nie, Songtao Xiang, Chiwei Chen

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yuanzhao Wang *The Seventh Clinical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, China.
Nana Chen *The First Clinical Medical School of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
Bangqiu ZhangThe Seventh Clinical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, China.
Pingping ZhuangThe Seventh Clinical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, China.
Bingtao TanThe Seventh Clinical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, China.
Changlong CaiThe Seventh Clinical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, China.
Niancai HeGuangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
Hao NieGuangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
Songtao XiangGuangdong Clinical Research Academy of Chinese Medicine; The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China. tonyxst@gzucm.edu.cn.
Chiwei ChenGuangdong Clinical Research Academy of Chinese Medicine; The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China. chenchiwei5542@163.com.

Funding

Basic and Applied Basic Research Foundation of Guangdong Province 2023A1515110654National Natural Science Foundation of China 82274512
6 · The paper itself

Abstract

Renal stones (RS) are common urologic condition with unclear pathogenesis. Role of aging-related differentially expressed genes (ARDEGs) in RS remains poorly understood. This study aims to identify potential aging-related biomarkers for RS, explore the functions of aging-associated genes, and investigate the immunological microenvironment in RS. ARDEGs were collected from the GEO, GeneCards, and Molecular Signatures databases. The roles of ARDEGs were analyzed using Gene Ontology (GO) enrichment analysis. Key genes were identified using machine learning methods. Immune infiltration in RS was assessed using the CIBERSORT and ssGSEA algorithms. A total of 22 ARDEGs were identified through analysis, including 9 up-regulated and 13 down-regulated genes. GO enrichment analysis revealed that these genes were mainly involved in RS-related biological processes such as macrophage proliferation and neuroinflammatory response. GSEA analysis showed that RS-associated genes were predominantly involved in immune regulation-related pathways. Using logistic regression, SVM, and LASSO regression algorithms, a successful early-diagnosis model for RS was developed, yielding 7 key genes: CNR1, KIT, HTR2A, DES, IL33, UCP2, and PPT1. Immunocyte infiltration analysis of RS samples showed that CD8 + T cells had the strongest positive correlation with M1 macrophages, while resting NK cells had the strongest negative correlation with activated NK cells. The DES gene showed the strongest positive correlation with resting mast cells, and the IL33 gene displayed the highest negative correlation with regulatory T cells. Bioinformatics analysis screened out 7 new potential markers for RS and explored the possible mechanism of RS senescence. These findings provide novel insights into the relationship between RS and senescence, as well as the diagnosis and treatment of RS, and enhance our understanding of the disease's occurrence and development mechanisms.

Indexed as

AgingBiomarkersComputational BiologyKidney CalculiDatabases, GeneticGene Expression ProfilingGene OntologyHumansBiomarkersBiomarkersImmune InfiltrationIntegrated BioinformaticsRenal Stone

Identifiers

PMID40593989
PMCPMC12214660

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.