Evidence map›Paper›PMID 40598565›Full record

ArticleParasites & vectors2025

SNP-based molecular diagnostic platform: rapid single-step identification of Theileria annulata and its buparvaquone-resistant strains.

Jin Che, Yijun Chai, Shuaiyang Zhao, Jinming Wang, Jianxun Luo, Guiquan Guan, Hong Yin, Wei Li

Abstract read
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In one paragraph

Article in Parasites & vectors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Jin Che *College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150006, China.
Yijun Chai *State Key Laboratory for Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Shuaiyang ZhaoState Key Laboratory for Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Jinming WangState Key Laboratory for Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Jianxun LuoState Key Laboratory for Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Guiquan GuanState Key Laboratory for Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. guanguiquan@caas.cn.
Hong YinState Key Laboratory for Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. yinhong@caas.cn.
Wei LiCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, 150006, China. neaulw@gmail.com.

Funding

National Key Research and Development Program of China 2022YFD1800200National Parasitic Resources Center NPRC-2019-194-30Natural Science Foundation of Gansu Province 22JR5RA031NBCITS CARS-37NSFC №31972701Special Project of Gansu Province 22CX8NA011the Innovation Program of Chinese Academy of Agricultural Sciences CAAS-ASTIP-2021-LVRIthe Science Fund for Creative Research Groups 22JR5RA024
6 · The paper itself

Abstract

backgroundTheileria annulata, a tick-borne protozoan that causes tropical theileriosis, poses a serious threat to livestock production in endemic regions. The emergence of resistance to buparvaquone, the primary chemotherapeutic treatment, has been attributed to acquired mutations in the cytochrome b (Cytb) gene, with identical resistance-associated polymorphisms observed in both laboratory-adapted strains and field isolates from China.

methodsA dual probe-specific real-time polymerase chain reaction (PCR) assay was developed to detect point mutations in the Cytb gene. The specificity, sensitivity, and reproducibility of the assay were validated, and its field applicability was evaluated via cattle blood samples (n = 531) collected from five endemic Chinese provinces.

resultsSix point mutations were identified in the Cytb gene, and the developed dual probe-specific real-time PCR assay simultaneously detected T. annulata infection and distinguished between the buparvaquone-sensitive and buparvaquone-resistant genotypes. The assay demonstrated a detection limit of 1 × 10

conclusionsThe developed dual probe-specific real-time PCR assay enables efficient detection of buparvaquone-resistant genotypes, providing important implications for guiding the treatment of tropical theileriosis and enhancing epidemiological surveillance of emerging resistance in endemic regions.

Indexed as

Antiprotozoal AgentsDrug ResistanceMolecular Diagnostic TechniquesNaphthoquinonesPolymorphism, Single NucleotideTheileria annulataTheileriasisAnimalsCattleCattle DiseasesChinaCytochromes bGenotypePoint MutationReal-Time Polymerase Chain ReactionReproducibility of ResultsAntiprotozoal AgentsbuparvaquoneCytochromes bNaphthoquinonesBuparvaquone resistanceCytochrome bReal-time PCRSNP genotypingTheileria annulata

Identifiers

PMID40598565
PMCPMC12219782

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.