Evidence map›Paper›PMID 40599651›Full record

ReviewFrontiers in cellular and infection microbiology2025

Extraction, purification, biological effects and applications of acrosin: a review.

Run Quan, Xiaolong Wu, Junjie Zhang, Rongxu Liu, Jianchun Han, Danyi Liu

Abstract readReview
In one paragraph

Review in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Garin3 regulates sperm-oocyte fusion and male fertility.Cellular and molecular life sciences : CMLS · 2026
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Run QuanCollege of Food Science, Northeast Agricultural University, Harbin, China.
Xiaolong WuCollege of Food Science, Northeast Agricultural University, Harbin, China.
Junjie ZhangCollege of Food Science, Northeast Agricultural University, Harbin, China.
Rongxu LiuHeilongjiang Institute of Green Food Science, Northeast Agricultural University, Harbin, China.
Jianchun HanCollege of Food Science, Northeast Agricultural University, Harbin, China.
Danyi LiuHeilongjiang Institute of Green Food Science, Northeast Agricultural University, Harbin, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Acrosin is a proteolytic enzyme in the sperm acrosome that can stimulate sperm to penetrate the zona pellucida, causing the fertilization of the oocyte. Its activity is a crucial indicator of the sperm's fertilization ability, which is critical in mammalian and human reproduction. However, there exists a lack of a comprehensive review of acrosin. In this study, we compared the extraction methods of acrosin, including acid extraction, buffer extraction, and saline extraction. The main methods for purifying acrosin, such as ammonium sulfate precipitation, ultrafiltration, dialysis, gel filtration chromatography, ion-exchange chromatography, and affinity chromatography, are reviewed. In addition, a detailed overview of the biological functions, inhibitors and applications of acrosin are outlined. This study provides methods for the extraction and purification of acrosin and some theoretical basis for the study of its properties. This provides a reference for further research on acrosin.

Indexed as

AcrosinAnimalsChromatography, Ion ExchangeHumansMaleSpermatozoaAcrosinacrosinacrosin inhibitorenzymatic propertiesextractionpurificationreproduction

Identifiers

PMID40599651
PMCPMC12209203

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.