Evidence map›Paper›PMID 40600821›Full record

ArticleDevelopment (Cambridge, England)2025

Deciphering the role of cis-regulatory elements and TFAP2C in the activation of zygotic Sox2 expression in mouse preimplantation embryos.

Jaehwan Kim, Chad S Driscoll, Lijia Li, Catherine A Wilson, Wei Xie, Jason G Knott

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Development (Cambridge, England), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jaehwan KimDevelopmental Epigenetics Laboratory, Department of Animal Science, Reproductive and Developmental Sciences Program, Michigan State University, East Lansing, MI 48824, USA.
Chad S DriscollDevelopmental Epigenetics Laboratory, Department of Animal Science, Reproductive and Developmental Sciences Program, Michigan State University, East Lansing, MI 48824, USA.
Lijia LiCenter for Stem Cell Biology and Regenerative Medicine, MOE Key Laboratory of Bioinformatics, New Cornerstone Science Laboratory, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Catherine A WilsonDevelopmental Epigenetics Laboratory, Department of Animal Science, Reproductive and Developmental Sciences Program, Michigan State University, East Lansing, MI 48824, USA.
Wei XieCenter for Stem Cell Biology and Regenerative Medicine, MOE Key Laboratory of Bioinformatics, New Cornerstone Science Laboratory, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Jason G KnottDevelopmental Epigenetics Laboratory, Department of Animal Science, Reproductive and Developmental Sciences Program, Michigan State University, East Lansing, MI 48824, USA.ORCID 0000-0003-1626-7933

Funding

Reproductive and Developmental Sciences Training Program - T32T32HD087166 · NICHD · MICHIGAN STATE UNIVERSITY · PI Ronald L Chandler, Keith E Latham · 2016 to 2026
$2.5M
Mechanisms of trophoblast lineage formation: Impact on postimplantation placental developmentR01HD095371 · NICHD · MICHIGAN STATE UNIVERSITY · PI KNOTT, JASON GLENN · 2018 to 2022
$1.6M
Michigan State UniversityNational Institute of Child Health and Human Development HD087166National Institute of Child Health and Human Development HD095371NICHD NIH HHS R01 HD095371NICHD NIH HHS T32 HD087166
6 · The paper itself

Abstract

Cell fate decisions in preimplantation embryos require the coordinated expression of pluripotency and lineage-specific transcription factors. SOX2 represents the first pluripotency regulator for which expression is restricted to the inside cells of mouse preimplantation embryos. However, the genetic mechanisms that activate the expression of zygotic Sox2 are poorly understood. Here, we report that Sox2 expression in mouse embryos is controlled by the actions of key cis-regulatory elements, including a proximal promoter and super enhancer. We show that TFAP2C, a key trophoblast lineage regulator, binds to the Sox2 proximal promoter to activate its expression. Lastly, we provide evidence that TFAP2C and the HIPPO signaling pathway cooperatively regulate Sox2 expression. In summary, this work has important implications for understanding how conventional trophoblast transcription factors, such as TFAP2C, contribute to the activation of early pluripotency genes to facilitate divergent cellular states that support lineage formation.

Indexed as

BlastocystGene Expression Regulation, DevelopmentalRegulatory Sequences, Nucleic AcidSOXB1 Transcription FactorsTranscription Factor AP-2ZygoteAnimalsFemaleHippo Signaling PathwayMicePromoter Regions, GeneticProtein Serine-Threonine KinasesSignal TransductionTrophoblastsProtein Serine-Threonine KinasesSox2 protein, mouseSOXB1 Transcription FactorsTfap2c protein, mouseTranscription Factor AP-2HIPPO signalingPluripotencyPreimplantation embryoSox2 regulatory regionsTFAP2C

Identifiers

PMID40600821
PMCPMC12338974

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.