Evidence map›Paper›PMID 40601221›Full record

ArticleDigestive diseases and sciences2025

Serum Hsa-miR-4695-5p Serves as a Novel Diagnostic Biomarker for Acute Severe Pancreatitis and Exacerbates Pathological Injury by Targeting SESN2.

Jiasui Chai, Jiejie Dong, Weixiao Hou, Jiayong Ning, Yan Liu, Yan Chen, Tong Zhang, Xingren Guo, Peng Yao

Abstract read
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Article in Digestive diseases and sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jiasui ChaiDepartment of Hepatobiliary Surgery, Yuncheng Central Hospital Affiliated to Shanxi Medical University, No. 3690, Hedong East Street, Yanhu District, Yuncheng, 044000, Shanxi Province, China.
Jiejie DongDepartment of Hepatobiliary Surgery, Yuncheng Central Hospital Affiliated to Shanxi Medical University, No. 3690, Hedong East Street, Yanhu District, Yuncheng, 044000, Shanxi Province, China.
Weixiao HouDepartment of Hepatobiliary Surgery, Yuncheng Central Hospital Affiliated to Shanxi Medical University, No. 3690, Hedong East Street, Yanhu District, Yuncheng, 044000, Shanxi Province, China.
Jiayong NingDepartment of Hepatobiliary Surgery, Yuncheng Central Hospital Affiliated to Shanxi Medical University, No. 3690, Hedong East Street, Yanhu District, Yuncheng, 044000, Shanxi Province, China.
Yan LiuDepartment of Hepatobiliary Surgery, Yuncheng Central Hospital Affiliated to Shanxi Medical University, No. 3690, Hedong East Street, Yanhu District, Yuncheng, 044000, Shanxi Province, China.
Yan ChenDepartment of Hepatobiliary Surgery, Yuncheng Central Hospital Affiliated to Shanxi Medical University, No. 3690, Hedong East Street, Yanhu District, Yuncheng, 044000, Shanxi Province, China.
Tong ZhangDepartment of Hepatobiliary Surgery, Yuncheng Central Hospital Affiliated to Shanxi Medical University, No. 3690, Hedong East Street, Yanhu District, Yuncheng, 044000, Shanxi Province, China.
Xingren GuoDepartment of Hepatobiliary Surgery, Yuncheng Central Hospital Affiliated to Shanxi Medical University, No. 3690, Hedong East Street, Yanhu District, Yuncheng, 044000, Shanxi Province, China.
Peng YaoDepartment of Hepatobiliary Surgery, Yuncheng Central Hospital Affiliated to Shanxi Medical University, No. 3690, Hedong East Street, Yanhu District, Yuncheng, 044000, Shanxi Province, China. yaopengyc@stu.xhu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveIncreasing evidence has revealed that aberrantly expressed miRNAs play important roles in stimulating pathogenesis and maintaining disease progression. However, the potential miRNAs involved in acute pancreatitis (AP) are still unclear. The present study aimed to explore the clinical significance of hsa-miR-4695-5p and its role behind AP progression.

methodSerum samples from 86 patients with AP (mild: 19 cases, moderate: 25 cases; severe: 42 cases) and 33 healthy cases were collected, and hsa-miR-4695-5p levels were detected by qRT-PCR analysis. Receiver operating characteristic (ROC) curves were employed to assess the diagnostic performance of hsa-miR-4695-5p. Caerulein-treated HPDE6-C7 cells were served as an in vitro cellular model for AP, cell viability, pro-inflammatory cytokines (TNF-α, IL-6 and IL-33) and oxidative stress were detected after silencing or overexpression of hsa-miR-4695-5p by CCK-8, ELISA, MDA and SOD kits. The influence of hsa-miR-4695-5p on macrophage polarization was verified through cell co-cultivation system by the detection of M1 and M2 macrophage markers. The targeting relationships between hsa-miR-4695-5p and sestrin 2 (SESN2) were confirmed by bioinformatics, luciferase reporter and RNA pull-down assays. In addition, a mouse AP model was used to confirm hsa-miR-4695-5p's role in AP.

resultsWe found that hsa-miR-4695-5p was upregulated in patients with AP relative to normal cases, and higher hsa-miR-4695-5p expression was observed in severe AP cases relative to mild and moderate AP cases. The combination of hsa-miR-4695-5p and serum amylase showed better diagnostic efficacy for severe AP than hsa-miR-4695-5p alone (AUC of 0.964 and 0.847). Interfering hsa-miR-4695-5p improved cell viability and increased SOD activity in caerulein-treated HPDE6-C7 cells, decreased MDA, TNF-α, IL-6 and IL-33 levels, and reduced the macrophage polarization toward M1. Reinforcing hsa-miR-4695-5p caused the opposite effects. SESN2 levels were downregulated in patients with severe AP and caerulein-treated HPDE6-C7 cells, and SESN2 was a direct target of hsa-miR-4695-5p. Moreover, SESN2 knockdown reversed the effects of interfering hsa-miR-4695-5p in caerulein-treated HPDE6-C7 cells. In vivo injection of hsa-miR-4695-5p antagomir attenuated pathological injury in AP mice.

conclusionUpregulation of serum hsa-miR-4695-5p could be a novel biomarker for diagnosis of severe AP, and hsa-miR-4695-5p may promote pathological injury in severe AP via targeting SESN2 expression.

Indexed as

MicroRNAsNuclear ProteinsPancreatitisAcute DiseaseAdultAnimalsBiomarkersCase-Control StudiesDisease ProgressionFemaleHumansMacrophagesMaleMiceMiddle AgedSestrinsBiomarkersMicroRNAsNuclear ProteinsSESN2 protein, humanSestrinsAPHas-miR-4695-5pOxidative stressPro-Inflammatory cytokinesSESN2

Identifiers

PMID40601221

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.