Evidence map›Paper›PMID 40610482›Full record

ArticleScientific reports2025

Effects of low glucose microenvironment on the proliferation, migration and senescence of meniscus-derived stem cells.

Jiabei Tong, Badr Alzahrani, Suresh Kumar Subbiah, Sanjiv Rampal, Rajesh Ramasamy, Zhijie Ma, Xiaoyun Wu, Pooi Ling Mok

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jiabei TongDepartment of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia.
Badr AlzahraniDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka, Saudi Arabia. baalzahrani@ju.edu.sa.
Suresh Kumar SubbiahCentre for Stem Cell Mediated Advanced Research Therapeutics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, 600077, India.
Sanjiv RampalDepartment of Orthopaedic and Traumatology, School of Medicine, International Medical University, 70300, Jalan Rasah, Seremban, Malaysia.
Rajesh RamasamyDepartment of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia.
Zhijie MaDepartment of Pharmacy, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China.
Xiaoyun WuDepartment of Technology, Inner Mongolia Stem Cell (ProterCell) Biotechnology Co., Ltd., Hohhot, China.
Pooi Ling MokDepartment of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia. pooi_ling@upm.edu.my.

Funding

GPI grant of Universiti Putra Malaysia GPI/2024/9798600GP-IPS grant of Universiti Putra Malaysia GP-IPS/2022/9718000National Key R&D Program of China 2023YFC2308200Natural Science Foundation of Inner Mongolia Autonomous Region 2025LHMS08003
6 · The paper itself

Abstract

Meniscus-derived stem cells (MeSCs) hold great promise for cytotherapy of the meniscus. Large-scale cell expansion in vitro is usually accompanied by decreased proliferation and migration and increased senescence, leading to a decrease in treatment efficiency. Therefore, the present study aimed to compare the effects of different concentrations of glucose on the proliferation, migration, senescence and protein expression of MeSCs. In this study, human MeSCs were cultured in two types of expansion media: low-glucose (LG) DMEM (5.5 mM glucose) and high-glucose (HG) DMEM (25 mM glucose). At specific passage number, the proliferation rate was evaluated by cell counting, the migration rate was evaluated by a scratch-wound assay, cell senescence was evaluated by β-GAL assays, and protein expression/phosphorylation was evaluated by ELISA. The results showed that a lower concentration of glucose promoted proliferation and migration, reduced cell senescence, and activated PI3K/Akt-related signaling pathways. This study provides a preliminary basis for the use of this medium composition for MeSC expansion.

Indexed as

Cell MovementCell ProliferationCellular SenescenceGlucoseMeniscusMesenchymal Stem CellsStem CellsCells, CulturedHumansPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSignal TransductionGlucosePhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktCell senescenceLow glucoseMeniscus-derived stem cellsMigrationProliferation

Identifiers

PMID40610482
PMCPMC12229553

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.