Evidence map›Paper›PMID 40615464›Full record

ArticleScientific reports2025

Genetic analysis of silique and seed traits in Brassica juncea (L.) Czern. under differential doses of nitrogen application.

Javed Akhatar, Anna Goyal, Meenakshi Mittal, Heena Verma, Kaur Gurpreet, Beerpal Kaur, Surinder S Banga, Chhaya Atri

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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Javed AkhatarDepartment of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab, 141004, India.
Anna GoyalDepartment of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab, 141004, India.
Meenakshi MittalDepartment of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab, 141004, India.
Heena VermaDepartment of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab, 141004, India.
Kaur GurpreetDepartment of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab, 141004, India.
Beerpal KaurDepartment of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab, 141004, India.
Surinder S BangaDepartment of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab, 141004, India.
Chhaya AtriDepartment of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab, 141004, India. chhayaatri@pau.edu.ORCID http://orcid.org/0000-0001-7187-7131

Funding

Department of Biotechnology, Ministry of Science and Technology, India BT/01/CEIB/12/I/03 Vol-I &Vol-II
6 · The paper itself

Abstract

Mustard, a major source of edible and industrial grade oils in the Indian subcontinent and various regions of Australia, Eastern Europe, and Canada, is also a protein resource for the animal feed industry. Silique and seed size are key traits for mustard improvement, but their inheritance mechanisms are not fully understood. We evaluated 92 inbred lines for silique length, seeds per silique, seed size, and rupture energy required to shatter a pod at three levels of nitrogen application in two crop seasons. Genotypes showed large phenotypic variations and a continuous distribution for all silique-related traits, suggesting a quantitative inheritance. Genotype × nitrogen interactions were significant for silique length, seeds per silique, and rupture energy. Association analysis identified 59 significant SNPs, whose annotations facilitated the prediction of 16 important genes underlying observed phenotypic variations. These genes are associated with silique formation (SHP2), grain formation (BG1, BG4), cell elongation (BRI1), grain filling (AT1G12500, AT1G77610, SWEET1, and AT3G14410), and silique shattering (UBP15, CO, INDEHISCENT, AGAMOUS1, FRUITFULL, and SHP2). RNA-seq data from 18 mustard genotypes revealed expression-level variations for identified candidate genes. Upregulation of SHP2 for SPS and resistance to silique shattering was observed, while downregulation of BG4 was observed in two genotypes with smallest seeds. This study provides valuable insight that may facilitate the marker-assisted selection (MAS) breeding for silique traits.

Indexed as

Mustard PlantNitrogenQuantitative Trait, HeritableSeedsGene Expression Regulation, PlantGenotypePhenotypePlant BreedingPolymorphism, Single NucleotideQuantitative Trait LociNitrogenCandidate genesGenome-wide association studiesPod shatteringRapeseed-mustardRNA-seqRupture energy

Identifiers

PMID40615464
PMCPMC12227704

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.