Evidence map›Paper›PMID 40620806›Full record

ArticleBio-protocol2025

Optimized Midgut Tissue Dissociation of Mosquitoes and Sandflies for High-Quality Single-Cell RNA Sequencing.

Ana Beatriz F Barletta, Octavio Augusto Talyuli, Pedro Cecilio, Carolina Barillas-Mury

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PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Ana Beatriz F BarlettaLaboratory of Malaria and Vector Research, National Institutes of Allergy and Infectious Diseases, National Institutes of Health; Rockville, MD, USA.
Octavio Augusto TalyuliLaboratory of Malaria and Vector Research, National Institutes of Allergy and Infectious Diseases, National Institutes of Health; Rockville, MD, USA.
Pedro CecilioLaboratory of Malaria and Vector Research, National Institutes of Allergy and Infectious Diseases, National Institutes of Health; Rockville, MD, USA.
Carolina Barillas-MuryLaboratory of Malaria and Vector Research, National Institutes of Allergy and Infectious Diseases, National Institutes of Health; Rockville, MD, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single-cell RNA sequencing has revolutionized molecular cell biology by enabling the identification of unique transcription profiles and cell transcription states within the same tissue. However, tissue dissociation presents a challenge for non-model organisms, as commercial kits are often incompatible, and current protocols rely on tissue enzymatic digestion for extended periods. Tissue digestion can alter cell transcription in response to temperature and the stress caused by enzymatic treatment. Here, we propose a protocol to stabilize RNA using a deep eutectic solvent (Vivophix, Rapid Labs) prior to tissue dissociation, thereby avoiding transcription changes induced by the process and preventing RNase activity during incubation. We validated this methodology for three medically important insect vectors:

Indexed as

Aedes aegyptiAnopheles gambiaeLutzomyia longipalpisMosquitoSand flySingle-cell RNA sequencingTissue dissociation

Identifiers

PMID40620806
PMCPMC12222636

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.