Evidence map›Paper›PMID 40628898›Full record

ArticleScientific reports2025

Optimizing extracellular matrix for endothelial differentiation using a design of experiments approach.

Mikayla L Hall, Wei-Han Lin, Brenda M Ogle

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Mikayla L HallDepartment of Biomedical Engineering, College of Science and Engineering, University of Minnesota, Minneapolis, MN, 55455, USA.
Wei-Han LinDepartment of Biomedical Engineering, College of Science and Engineering, University of Minnesota, Minneapolis, MN, 55455, USA.
Brenda M OgleDepartment of Biomedical Engineering, College of Science and Engineering, University of Minnesota, Minneapolis, MN, 55455, USA. ogle@umn.edu.

Funding

TRAINING-PULMONARY CELL &MOLECULAR BIOLOGY &PHYSIOLOGYT32HL007741 · NHLBI · UNIVERSITY OF MINNESOTA TWIN CITIES · PI DUDLEY, R. ADAMS, INGBAR, DAVID H · 1994 to 2023
$13.9M
Epicardial regulation of cardiomyocyte function via modulation of extracellular signals: toward a model of human muscle pump functionR01HL160779 · NHLBI · UNIVERSITY OF MINNESOTA · PI OGLE, BRENDA M · 2022 to 2025
$1.9M
Extracellular matrix regulation of differentiation via modulation of ILK: application to 3D bioprinting of cardiac tissueR01HL137204 · NHLBI · UNIVERSITY OF MINNESOTA · PI MCALPINE, MICHAEL, OGLE, BRENDA M · 2017 to 2020
$1.7M
National Heart and Lung Institute HL160779NHLBI NIH HHS HL007741NHLBI NIH HHS HL137204NHLBI NIH HHS R01 HL137204NHLBI NIH HHS R01 HL160779NHLBI NIH HHS T32 HL007741
6 · The paper itself

Abstract

The extracellular matrix (ECM) plays a vital role in stem cell differentiation to endothelial cells in vivo and is also important for the specification of endothelial cells in vitro. Individual ECM components have previously been shown to support endothelial differentiation; here, we use a Design of Experiments approach to optimize ECM composition to more effectively drive endothelial differentiation. We found that a combination of Collagen I, Collagen IV, and Laminin 411 could induce endothelial differentiation well beyond that found with Matrigel, the most commonly used differentiation substrate for endothelial cells. We also show that the addition of vascular endothelial growth factor (VEGF) during differentiation improves outcomes and that transforming growth factor beta (TGFβ) inhibits specification. The optimized ECM formulation (EO) was subsequently used to create bioprinted constructs, demonstrating its ability to spatially define endothelial differentiation in 3D environments. Our results build our mechanistic knowledge of the signaling axes that regulate differentiation in response to ECM stimulation with practical implications for the vascularization of engineered tissues.

Indexed as

Cell DifferentiationEndothelial CellsExtracellular MatrixAnimalsCells, CulturedCollagenHumansHuman Umbilical Vein Endothelial CellsLamininTissue EngineeringTransforming Growth Factor betaVascular Endothelial Growth Factor ACollagenLamininTransforming Growth Factor betaVascular Endothelial Growth Factor A

Identifiers

PMID40628898
PMCPMC12238403

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.