Evidence mapPaperPMID 40629475Full record

ArticleEuropean journal of medical research2025

Trophoblast cell response to periodontal pathogens unveils oxidative stress networks between periodontitis and pre-eclampsia: Mendelian randomization, transcriptomic analysis, and cellular experimental validation.

Lihua Hu, Shuqin Liu

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Article in European journal of medical research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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2 authors.

Lihua HuDepartment of Maternal Health Care, Xinyu Municipal Maternity and Child Care Hospital, Laodong South Road No. 292, Xinyu, 338025, Jiangxi Province, China.
Shuqin LiuDepartment of Stomatology, Jiangxi Provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College, Aiguo Road No. 152, Nanchang, 330006, Jiangxi Province, China. lsq200109006@163.com.

Funding

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6 · The paper itself

Abstract

backgroundPeriodontitis has been associated with an increased risk of pre-eclampsia, but the underlying mechanisms and causal relationship remain unclear. Oxidative stress has been implicated in both conditions, suggesting a potential mechanistic link.

objectivesTo investigate the causal relationship between periodontitis and pre-eclampsia and elucidate shared molecular pathways, with a focus on oxidative stress-related mechanisms.

methodsWe employed a multi-faceted approach combining Mendelian Randomization (MR) analysis, transcriptomic data mining, and in vitro experiments. MR analysis used genome-wide association study data to assess causality. Transcriptomic analysis of public data sets identified cross-talk genes and oxidative stress markers. In vitro experiments using HTR-8/SVneo trophoblast cells treated with Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) validated key findings.

resultsMR analysis revealed no significant causal relationship between periodontitis and pre-eclampsia. Transcriptomic analysis identified 31 cross-talk genes, including 10 periodontitis and pre-eclampsia (PD&PE) marker genes and 5 oxidative stress (OS) marker genes. In vitro experiments showed increased expression of OS marker genes (ARG1, FZD1, GCLC, NQO1, and TP53INP1) and elevated reactive oxygen species levels in Pg-LPS-treated trophoblast cells. Antioxidant enzymes (SOD1, CAT, Nrf2, and HO-1) and oxidase (NOX2) were also upregulated, indicating oxidative stress response.

conclusionsWhile no direct causal relationship was found, this study reveals shared molecular pathways between periodontitis and pre-eclampsia, particularly through oxidative stress mechanisms. These findings provide new insights into the periodontitis-pre-eclampsia relationship and identify potential targets for therapeutic interventions and biomarker development.

Indexed as

Oxidative StressPeriodontitisPre-EclampsiaTrophoblastsFemaleGene Expression ProfilingGenome-Wide Association StudyHumansMendelian Randomization AnalysisPorphyromonas gingivalisPregnancyTranscriptomeOxidative stressPeriodontitisPre-eclampsiaTranscriptomicsTrophoblast cells

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PMID40629475
PMCPMC12236035

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