Evidence map›Paper›PMID 40630939›Full record

ArticleFrontiers in parasitology2025

Down-regulation of colon mucin production induced by

Yulia Dwi Setia, Mio Kokubo-Tanaka, Ryusei Tanaka, Akemi Yoshida, Eiji Nagayasu, Parnian Ahmadi, Ayako Yoshida, Haruhiko Maruyama

Abstract read
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In one paragraph

Article in Frontiers in parasitology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yulia Dwi SetiaDivision of Parasitology, Department of Infectious Disease, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
Mio Kokubo-TanakaDivision of Parasitology, Department of Infectious Disease, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
Ryusei TanakaDivision of Parasitology, Department of Infectious Disease, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
Akemi YoshidaLaboratory of Genomics, Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan.
Eiji NagayasuDivision of Parasitology, Department of Infectious Disease, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
Parnian AhmadiCenter of Animal Disease Control, University of Miyazaki, Miyazaki, Japan.
Ayako YoshidaCenter of Animal Disease Control, University of Miyazaki, Miyazaki, Japan.
Haruhiko MaruyamaDivision of Parasitology, Department of Infectious Disease, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Methods: Male C57BL/6 mice were orally infected with 300 oocysts. Fecal oocyst shedding and body weight were monitored daily. Colon tissues were collected at 3, 8, and 13 days post-infection (dpi) to assess pathological changes. Parasite burden was assessed by histological analysis (H&E staining) and qPCR targeting 5S rRNA. Goblet cells were visualized using PAS-Alcian Blue staining and Muc2 immunohistochemistry. To elucidate mechanisms of goblet cell dysfunction, we performed RNA sequencing of large intestine tissue to examine host as well as parasite transcriptomes. Results: Fecal oocyst excretion peaked at 8-9 dpi. Body weight decreased from 6 to 11 dpi, with recovery after 12 dpi. Maximal parasite accumulation in the proximal colon was observed at 8 dpi in histological examination as well as qPCR. Colon length was significantly shortened at 3 dpi. Goblet cell area significantly reduced at 8 dpi (p < 0.05). RNA sequencing of infected large intestines revealed that Discussion: Our findings suggested that

Indexed as

colitisEimeria pragensisgoblet cell dysfunctionmouse modelmucin down-regulation

Identifiers

PMID40630939
PMCPMC12234464

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.