Evidence map›Paper›PMID 40631095›Full record

ArticlebioRxiv : the preprint server for biology2025

Sideroflexins enable mitochondrial transport of polar neutral amino acids.

Samuel Block, Fangtao Chi, Paul C Rosen, S Sebastian Pineda, Alicia M Darnell, Keene L Abbott, Izabella A Pena, Myriam Heiman, Ömer H Yilmaz, Nora Kory and 1 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Samuel BlockDavid H. Koch Institute for Integrative Cancer Research, MIT, Cambridge, MA, USA.ORCID 0009-0003-3998-0122
Fangtao ChiDavid H. Koch Institute for Integrative Cancer Research, MIT, Cambridge, MA, USA.
Paul C RosenDepartment of Biology, MIT, Cambridge, MA, USA.
S Sebastian PinedaDepartment of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.ORCID 0000-0002-8903-0550
Alicia M DarnellDavid H. Koch Institute for Integrative Cancer Research, MIT, Cambridge, MA, USA.
Keene L AbbottDavid H. Koch Institute for Integrative Cancer Research, MIT, Cambridge, MA, USA.
Izabella A PenaPicower Institute for Learning and Memory, MIT, Cambridge, MA, USA.
Myriam HeimanPicower Institute for Learning and Memory, MIT, Cambridge, MA, USA.
Ömer H YilmazDavid H. Koch Institute for Integrative Cancer Research, MIT, Cambridge, MA, USA.
Nora KoryDepartment of Molecular Metabolism, Harvard T.H. Chan School of Public Health, Boston, MA, USA.ORCID 0000-0001-5307-9021
Matthew G Vander HeidenDavid H. Koch Institute for Integrative Cancer Research, MIT, Cambridge, MA, USA.ORCID 0000-0002-6702-4192

Funding

VIRUS PRODUCTION COREP30CA014051 · NCI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI Jacqueline A. Lees · 1985 to 2026
$93.9M
PRE-DOCTORAL TRAINING IN THE BIOLOGICAL SCIENCEST32GM007287 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BELL, STEPHEN P., KEATING, AMY E · 1985 to 2019
$40.8M
Training Program in Molecular and Integrative Physiological SciencesT32HL007118 · NHLBI · HARVARD UNIVERSITY (SCH OF PUBLIC HLTH) · PI LU, QUAN · 1985 to 2025
$14.3M
Understanding the role of metabolism in cancerR35CA242379 · NCI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI VANDER HEIDEN, MATTHEW G. · 2019 to 2025
$5.8M
Molecular Mechanisms Underlying Cell Type-Specific Vulnerability in Huntington’s DiseaseR35NS127327 · NINDS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI Myriam Heiman · 2022 to 2026
$2.7M
Substrates and functions of the Sideroflexin mitochondrial transporter familyR00CA241332 · NCI · HARVARD SCHOOL OF PUBLIC HEALTH · PI KORY, NORA · 2021 to 2023
$669k
Exploiting metabolic vulnerabilities of breast cancer brain metastases for therapyF31CA271787 · NCI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI ABBOTT, KEENE · 2022 to 2023
$94k
NCI NIH HHS F31 CA271787NCI NIH HHS P30 CA014051NCI NIH HHS R00 CA241332NCI NIH HHS R35 CA242379NHLBI NIH HHS T32 HL007118NIGMS NIH HHS T32 GM007287NINDS NIH HHS R35 NS127327
6 · The paper itself

Abstract

Mitochondria contribute to compartmentalized metabolism in eukaryotic cells, supporting key enzymatic reactions for cell function and energy homeostasis. However, this compartmentalization necessitates regulated metabolite transport across mitochondrial membranes. Although many transport proteins have been identified, several mitochondrial amino acid transporters remain largely uncharacterized. Using CRISPR-Cas9-mediated candidate transporter knockouts coupled with assessment of metabolite transport via a mitochondrial swelling assay, we identify SFXN1, previously characterized for its role in mitochondrial serine transport, as a protein that mediates mitochondrial transport of a range of other polar neutral amino acids including proline, glycine, threonine, taurine, hypotaurine, β-alanine, and γ-aminobutyric acid (GABA). Furthermore, the SFXN1 paralogues SFXN2 and SFXN3 partially complement loss of SFXN1 to enable glycine transport, while SFXN2 and SFXN5 partially complement loss of SFXN1 to enable GABA transport. Altogether, these data suggest that sideroflexins facilitate the transport of polar neutral amino acids across the inner mitochondrial membrane.

Identifiers

PMID40631095
PMCPMC12236812

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.