Evidence map›Paper›PMID 40640417›Full record

ArticleScientific reports2025

Development of a direct whole genome sequencing for hepatitis A virus from serum and analysis of genetic characteristics.

Daseul Yeo, Soontag Jung, Danbi Yoon, Seongwon Hwang, Dong Jae Lim, Songfeng Jin, Jinho Choi, Ki Ho Hong, Changsun Choi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Daseul YeoDepartment of Food and Nutrition, School of Food Science and Technology, College of Biotechnology and Natural Resources, Chung-Ang University, 4726 Seodongdaero, Daeduck-myeon, Anseong-si, Gyeonggi-do, 17546, Republic of Korea.
Soontag JungDepartment of Food and Nutrition, School of Food Science and Technology, College of Biotechnology and Natural Resources, Chung-Ang University, 4726 Seodongdaero, Daeduck-myeon, Anseong-si, Gyeonggi-do, 17546, Republic of Korea.
Danbi YoonDepartment of Food and Nutrition, School of Food Science and Technology, College of Biotechnology and Natural Resources, Chung-Ang University, 4726 Seodongdaero, Daeduck-myeon, Anseong-si, Gyeonggi-do, 17546, Republic of Korea.
Seongwon HwangDepartment of Food and Nutrition, School of Food Science and Technology, College of Biotechnology and Natural Resources, Chung-Ang University, 4726 Seodongdaero, Daeduck-myeon, Anseong-si, Gyeonggi-do, 17546, Republic of Korea.
Dong Jae LimDepartment of Food and Nutrition, School of Food Science and Technology, College of Biotechnology and Natural Resources, Chung-Ang University, 4726 Seodongdaero, Daeduck-myeon, Anseong-si, Gyeonggi-do, 17546, Republic of Korea.
Songfeng JinDepartment of Food and Nutrition, School of Food Science and Technology, College of Biotechnology and Natural Resources, Chung-Ang University, 4726 Seodongdaero, Daeduck-myeon, Anseong-si, Gyeonggi-do, 17546, Republic of Korea.
Jinho ChoiSanigen, 16 Heungan-daero, Dongan-gu, Anyang-si, Gyeonggi-do, 14059, Republic of Korea.
Ki Ho HongDepartment of Laboratory Medicine, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.
Changsun ChoiDepartment of Food and Nutrition, School of Food Science and Technology, College of Biotechnology and Natural Resources, Chung-Ang University, 4726 Seodongdaero, Daeduck-myeon, Anseong-si, Gyeonggi-do, 17546, Republic of Korea. cchoi@cau.ac.kr.

Funding

the Ministry of Food and Drug Safety 20162MFDS033
6 · The paper itself

Abstract

Hepatitis A virus (HAV) is transmitted via the fecal-oral route, including through person-to-person contact and consumption of contaminated food. In 2019, 17,638 cases of HAV infection were reported in South Korea. The use of whole-genome sequencing (WGS) for rapid and accurate epidemiological investigation of HAV remains challenging due to the low viral titers and the lack of a cell culture system. Here, we developed a multiplex PCR (mPCR)-based next generation sequencing (NGS) method for direct WGS of HAV from serum using the Illumina platform. Overlapping primers were designed to generate amplicons covering the entire HAV genome, enabling successful sequencing from samples with viral titers as low as 3.0 log

Indexed as

Genome, ViralHepatitis AHepatitis A virusWhole Genome SequencingGenotypeHigh-Throughput Nucleotide SequencingHumansMultiplex Polymerase Chain ReactionPhylogenyRepublic of KoreaDendrogram analysisHepatitis A virusIllumina platformPhylogenyWhole-genome sequencing

Identifiers

PMID40640417
PMCPMC12246155

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.