Evidence mapPaperPMID 40642947Full record

ArticleImmunity, inflammation and disease2025

Leucine-Rich Repeat Containing 15 Promotes the Inflammatory Response in Rheumatoid Arthritis by Regulating NF-κB Pathway.

Miaomiao Xin, Guangtao Xia, Xin Guan, Guangmin Xi, Min Fu

Abstract read
In one paragraph

Article in Immunity, inflammation and disease, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Miaomiao XinDepartment of Rheumatology and Immunology, Shandong Provincial Hospital Affiliated to Shandong First Medical University (Shandong Provincial Hospital), Jinan, Shandong, China.
Guangtao XiaDepartment of Rheumatology and Immunology, Shandong Provincial Hospital Affiliated to Shandong First Medical University (Shandong Provincial Hospital), Jinan, Shandong, China.
Xin GuanDepartment of Rheumatology and Immunology, Shandong Provincial Hospital Affiliated to Shandong First Medical University (Shandong Provincial Hospital), Jinan, Shandong, China.
Guangmin XiCollege of Life Sciences, Qilu Normal University, Jinan, Shandong, China.
Min FuDepartment of Rheumatology and Immunology, Shandong Provincial Hospital Affiliated to Shandong First Medical University (Shandong Provincial Hospital), Jinan, Shandong, China.ORCID 0009-0006-7876-3034

Funding

This study was supported by the Natural Science Foundation of Shandong Province (ZR2021MH265), the Clinical Medical Science and Technology Innovation Program (202019051), and the National Natural Science Foundation of China (No. 82001744).
6 · The paper itself

Abstract

purposeTo explore the influence and molecular mechanism of leucine-rich repeat containing 15 (LRRC15) in rheumatoid arthritis (RA) model induced by collagen-induced arthritis (CIA) in rats and interleukin-1 beta (IL-1β) treated fibroblast-like synoviocytes (FLSs).

methodsLRRC15 expression was analyzed using reverse transcription quantitative polymerase chain reaction (RT-qPCR), western blot analysis, and immunohistochemistry. Hematoxylin-eosin (H&E) and safranin-O-green staining were performed to assess the pathological changes in the joint tissues of rats. The messenger ribonucleic acid (mRNA) and protein expression of interferon gamma (IFN-γ), interleukin (IL)-6, IL-1β, and IL-10 was detected by RT-qPCR, enzyme-linked immunosorbent assay (ELISA), and western blot. Cell proliferation and migration was surveyed using cell counting kit-8 (CCK-8), 5-Ethynyl-2'-deoxyuridine (EdU), and Transwell assays. Western blot was applied to detect the protein changes of nuclear factor kappaB (NF-κB) subunit p65, phosphorylated (p)-p65, inhibitor of kappa Bα (IκBα), phosphorylated inhibitor kappa Bα (p-IκBα), and nuclear factor erythroid 2-related factor 2 (Nrf2).

resultsIn CIA rats and IL-1β-treated FLSs, LRRC15 expression was upregulated. In vivo, Lrrc15 silencing prevented joint damage, inhibited the expression of IFN-γ, IL-6, IL-1β, and p65, and increased the expression of IL-10 and IκBα. In vitro, Lrrc15 silencing inhibited the proliferation, migration, and the expression of IFN-γ, IL-6, IL-1β, p-p65, and p-IκBα, and increased the expression of IL-10, IκBα, and Nrf2 in FLSs.

conclusionLrrc15 silencing relieved joint damage and inflammatory response in RA, and this may be associated with the inhibition of the NF-κB pathway.

Indexed as

Arthritis, ExperimentalArthritis, RheumatoidInflammationNF-kappa BAnimalsCell MovementCell ProliferationCells, CulturedCytokinesMaleRatsRats, Sprague-DawleySignal TransductionSynoviocytesCytokinesNF-kappa Bfibroblast‐like synoviocytesinflammatory responseLrrc15NF‐κB pathwayrheumatoid arthritis

Identifiers

PMID40642947
PMCPMC12246832

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.