Evidence map›Paper›PMID 40643339›Full record

ArticleInvestigative ophthalmology & visual science2025

Elastin as a Novel Extracellular Matrix From Aberrant HDAC4 Expression in PDGF-BB-Induced Orbital Fibroblasts From Graves' Ophthalmopathy Patients.

Rajit Chompoowong, Jutamas Wongphoom, Nakarin Kitkumthorn, Preamjit Saonanon, Vannakorn Pruksakorn, Tanapat Palaga, Nattiya Hirankarn, P Martin van Hagen, Willem A Dik, Sita Virakul

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Investigative ophthalmology & visual science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Rajit ChompoowongMedical Microbiology, Interdisciplinary Program, Graduate School, Chulalongkorn University, Bangkok, Thailand.
Jutamas WongphoomDepartment of Pathology, King Chulalongkorn Memorial Hospital, Bangkok, Thailand.
Nakarin KitkumthornDepartment of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
Preamjit SaonanonDepartment of Ophthalmology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Vannakorn PruksakornDepartment of Ophthalmology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Tanapat PalagaDepartment of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
Nattiya HirankarnCenter of Excellence in Immunology and Immune-mediated Disease, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
P Martin van HagenCenter of Excellence in Immunology and Immune-mediated Disease, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Willem A DikLaboratory Medical Immunology, Department of Immunology, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.
Sita VirakulDepartment of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Purpose: The purpose of this study was to investigate fibroblast markers and histone deacetylase (HDAC) 4 in orbital tissues and orbital fibroblasts from patients with Graves' ophthalmopathy (GO) and healthy controls. Methods: Hematoxylin and eosin (H&E), Masson's trichrome, and Verhoeff's Van Gieson (VVG) staining were performed on GO and control orbital tissues. Immunohistochemistry on fibroblast markers were investigated. GO orbital fibroblasts (GOFs) and control orbital fibroblasts (COFs) stimulated with platelet-derived growth factor (PDGF)-BB were assessed for collagen type I alpha I (COL1A1), elastin (ELN), and fibrillin-2 (FBN2) mRNA expression. HDAC4 knockdown in GOF and COF were assessed to study its impact on elastin expression. GO orbital tissues and GOF treated with either LMK-235 or tasquinimod were examined. Results: Orbital tissues exhibited accumulation of adipocytes, orbital fibroblasts, collagen, and elastin. After 2 hours, PDGF-BB stimulation induced an 8-fold increase of ELN in GOF and a 5-fold increase of ELN in COF compared to no treatment, whereas COL1A1 and FBN2 mRNA levels were later induced after 24 hours. Elastin protein expression was significantly higher in GOF compared to COF at both the basal level and after PDGF-BB stimulation. HDAC4 knockdown significantly reduced PDGF-BB-induced ELN mRNA expression in GOF but not in COF. LMK-235 inhibited ELN mRNA expression in GOF. However, LMK-235 and tasquinimod did not decrease ELN mRNA level in GO orbital tissues. Conclusions: Orbital tissues exhibit shared and unique characteristics from other tissues. Our in vitro studies showed that elastin mRNA and protein expression increased in response to PDGF-BB stimulation in GOF which might be due to aberrant HDAC4 levels.

Indexed as

ElastinFibroblastsGraves OphthalmopathyHistone DeacetylasesRepressor ProteinsAdultAgedAged, 80 and overBecaplerminBenzamidesCase-Control StudiesDecompression, SurgicalFemaleGene Expression RegulationGene Knockdown TechniquesHistone Deacetylase InhibitorsBecaplerminBenzamidesElastinHDAC4 protein, humanHistone Deacetylase InhibitorsHistone DeacetylasesLMK-235QuinolonesRepressor Proteinstasquinimod

Identifiers

PMID40643339
PMCPMC12266294

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.