ArticleCell reports methods2025
Deuterium labeling enables proteome-wide turnover kinetics analysis in cell culture.
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Article in Cell reports methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6citing papers in PubMed
–field-weighted citation impact
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- Kinetic Lipidomics: QuantifyingbioRxiv : the preprint server for biology · 2026Article
- Heavy water labeling reveals metabolic flexibility of amino acid and polyamine pathways in mammalian cells.Communications chemistry · 2026Article
- A Computational Model for Determining Labeling Duration in Protein Turnover Studies Using a Single Deuterated Water Labeled Sample.Journal of the American Society for Mass Spectrometry · 2026Article
- Protocol to measure protein half-life in cell culture using heavy water.STAR protocols · 2026Article
- Duplexing metabolic deuterated water-labeled samples using dimethyl labeling to estimate protein turnover rates.Communications chemistry · 2025Article
- Improved Method to Determine Protein Turnover Rates with Heavy Water Labeling by Mass Isotopomer Ratio Selection.Journal of proteome research · 2025Article
Corrections and comments
- Update of
Authors and funding
12 authors.
Lorena AlamilloDepartment of Medicine, Division of Cardiology, University of Colorado School of Medicine, Aurora, CO, USA; Consortium for Fibrosis Research & Translation, University of Colorado School of Medicine, Aurora, CO, USA.
Dominic C M NgDepartment of Medicine, Division of Cardiology, University of Colorado School of Medicine, Aurora, CO, USA; Consortium for Fibrosis Research & Translation, University of Colorado School of Medicine, Aurora, CO, USA.
Jordan CurrieDepartment of Medicine, Division of Cardiology, University of Colorado School of Medicine, Aurora, CO, USA; Consortium for Fibrosis Research & Translation, University of Colorado School of Medicine, Aurora, CO, USA.
Alexander BlackDepartment of Medicine, Division of Cardiology, University of Colorado School of Medicine, Aurora, CO, USA; Consortium for Fibrosis Research & Translation, University of Colorado School of Medicine, Aurora, CO, USA.
Boomathi PandiDepartment of Medicine, Division of Cardiology, University of Colorado School of Medicine, Aurora, CO, USA; Consortium for Fibrosis Research & Translation, University of Colorado School of Medicine, Aurora, CO, USA.
Vyshnavi MandaDepartment of Medicine, Division of Cardiology, University of Colorado School of Medicine, Aurora, CO, USA; Consortium for Fibrosis Research & Translation, University of Colorado School of Medicine, Aurora, CO, USA.
Jay PavelkaDepartment of Medicine, Division of Cardiology, University of Colorado School of Medicine, Aurora, CO, USA; Consortium for Fibrosis Research & Translation, University of Colorado School of Medicine, Aurora, CO, USA.
Peyton SchaalDepartment of Medicine, Division of Cardiology, University of Colorado School of Medicine, Aurora, CO, USA; Consortium for Fibrosis Research & Translation, University of Colorado School of Medicine, Aurora, CO, USA.
Joshua G TraversDepartment of Medicine, Division of Cardiology, University of Colorado School of Medicine, Aurora, CO, USA; Consortium for Fibrosis Research & Translation, University of Colorado School of Medicine, Aurora, CO, USA.
Timothy A McKinseyDepartment of Medicine, Division of Cardiology, University of Colorado School of Medicine, Aurora, CO, USA; Consortium for Fibrosis Research & Translation, University of Colorado School of Medicine, Aurora, CO, USA.
Maggie P Y LamDepartment of Medicine, Division of Cardiology, University of Colorado School of Medicine, Aurora, CO, USA; Consortium for Fibrosis Research & Translation, University of Colorado School of Medicine, Aurora, CO, USA; Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO, USA. Electronic address: maggie.lam@cuanschutz.edu.
Edward LauDepartment of Medicine, Division of Cardiology, University of Colorado School of Medicine, Aurora, CO, USA; Consortium for Fibrosis Research & Translation, University of Colorado School of Medicine, Aurora, CO, USA. Electronic address: edward.lau@cuanschutz.edu.
Funding
Cellular and Molecular Biology at MichiganT32GM145470 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI John Chadwick Brenner · 2022 to 2026
$4.1MAlternative Protein Isoforms in Ventricular RemodelingR01HL141278 · NHLBI · UNIVERSITY OF COLORADO DENVER · PI Maggie Lam · 2018 to 2026
$3.5MPost-transcriptional regulations of proteomes in stress and senescenceR01GM144456 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI LAM, MAGGIE · 2022 to 2025
$2.2MInvestigations of Proteome Turnover Kinetics Under Cellular DifferentiationR35GM146815 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI Edward Lau · 2022 to 2026
$2.1M15-PGDH-Mediated Eicosanoid Degradation in Cardiac Fibrosis and Heart FailureR01HL171711 · NHLBI · UNIVERSITY OF COLORADO DENVER · PI Timothy McKinsey · 2024 to 2026
$1.6MMulti-Omics Approach to Identify Cardiokines in Human iPSC ModelsR00HL144829 · NHLBI · UNIVERSITY OF COLORADO DENVER · PI LAU, EDWARD · 2020 to 2022
$733kElucidating the Molecular Mechanisms and Cellular Specificity of HDAC Inhibitor Efficacy in Diastolic DysfunctionK99HL166708 · NHLBI · UNIVERSITY OF COLORADO DENVER · PI TRAVERS, JOSHUA · 2023 to 2024
$214kNHLBI NIH HHS K99 HL166708NHLBI NIH HHS R00 HL144829NHLBI NIH HHS R01 HL141278NHLBI NIH HHS R01 HL171711NIGMS NIH HHS R01 GM144456NIGMS NIH HHS R35 GM146815NIGMS NIH HHS T32 GM145470
Abstract
Protein turnover is a critical component of gene expression regulation and cellular homeostasis, yet methods for measuring turnover rates that are scalable and applicable to different models are still needed. We introduce an improved D
Indexed as
Cell Culture TechniquesDeuteriumProteomeProteomicsCell DifferentiationCells, CulturedFibroblastsHumansInduced Pluripotent Stem CellsKineticsMyocytes, CardiacDeuteriumProteomeCP: molecular biologyCP: systems biologydegronheavy waterhiPSCmass spectrometryprotein synthesisprotein turnoversecretometurnover kinetics
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LicenceCC BY-NC-ND
Read underepoch 390
Registered trials
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.