Electrochemical Determination of Creatinine Based on Multienzyme Cascade-Modified Nafion/Gold Nanoparticles/Screen-Printed Carbon Composite Biosensors.

Jialin Yang, Ruizhi Yu, Wanxin Zhang, Yijia Wang, Zejun Deng

Abstract read

In one paragraph

Article in Sensors (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jialin YangSchool of Materials Science and Engineering, State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China.

Ruizhi YuSchool of Materials Science and Engineering, State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China.

Wanxin ZhangSchool of Materials Science and Engineering, State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China.

Yijia WangHunan Key Laboratory for Super Microstructure and Ultrafast Process, School of Physics, Central South University, Changsha 410083, China.ORCID 0009-0008-8710-1379

Zejun DengSchool of Materials Science and Engineering, State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China.ORCID 0000-0002-4069-6896

Funding

Natural Science Foundation of Hunan Province No. 2023JJ40722Natural Science Foundation of Hunan Province No. 2024JJ6511

6 · The paper itself

Abstract

Creatinine serves as a crucial diagnostic biomarker for assessing kidney disease. This work developed portable non-enzymatic and multienzyme-modified electrochemical biosensors for the detection of creatinine based on commercial screen-printed carbon electrodes (SPCEs). The non-enzymatic creatinine sensor was constructed by the electrochemical deposition of AuNPs onto the surface of a pre-activated SPCE by electrochemical activation, followed by the surface modification of a Nafion membrane. The developed AuNPs/SCPE exhibited excellent reproducibility, and the proposed Nafion/AuNPs/SPCE sensor showed excellent detection sensitivity and selectivity toward creatinine. In comparison, the enzymatic creatinine biosensor was gradually established by the electrodeposition of a Prussian blue (PB) membrane on the optimal AuNPs/SCPE surface, followed by multi-enzyme cascade modification (which consisted of creatinine amidohydrolase (CA), creatine oxidase (CI) and sarcosine oxidase (SOx)) and drop-casting the Nafion membrane to stabilize the interface. The introduction of a PB interlayer acted as the redox layer to monitor the generation of hydrogen peroxide (H

Indexed as

Biosensing TechniquesCreatinineElectrochemical TechniquesGoldMetal NanoparticlesCarbonElectrodesFluorocarbon PolymersHumansHydrogen PeroxideSarcosine OxidaseCarbonCreatinineFluorocarbon PolymersGoldHydrogen Peroxideperfluorosulfonic acidSarcosine Oxidasecreatinineelectrochemical biosensorgold nanoparticlesmulti-enzyme cascade modification

Identifiers

PMID40648387

DOI10.3390/s25134132

PMCPMC12251950

What Socratic holds

Textmetadata

LicenceCC BY

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.