Evidence map›Paper›PMID 40649903›Full record

ArticleInternational journal of molecular sciences2025

On-Line Preconcentration of Selected Kynurenine Pathway Metabolites and Amino Acids in Urine via Pressure-Assisted Electrokinetic Injection in a Mixed Micelle System.

Michał Pieckowski, Ilona Olędzka, Tomasz Bączek, Piotr Kowalski

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Michał PieckowskiDepartment of Pharmaceutical Chemistry, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland.ORCID 0000-0002-0467-1560
Ilona OlędzkaDepartment of Pharmaceutical Chemistry, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland.ORCID 0000-0001-8898-6991
Tomasz BączekDepartment of Pharmaceutical Chemistry, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland.ORCID 0000-0001-6468-1974
Piotr KowalskiDepartment of Pharmaceutical Chemistry, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland.ORCID 0000-0002-8446-8238

Funding

The authors gratefully acknowledge the support from the National Center for Research and Development in Poland (Agreement No. V4-KOREA/2/9/ATBG/2024) for the project entitled: "Development of High Sensitivity and High Resolution Analytical Tools for the B V4-KOREA/2/9/ATBG/2024
6 · The paper itself

Abstract

To enhance the signal intensity of kynurenines, which are present at trace concentrations in biological fluids, a novel analytical approach was developed, combining pressure-assisted electrokinetic injection (PAEKI) with a mixed micelle system based on sodium dodecyl sulfate (SDS) and Brij-35. The method was applied to key compounds of the kynurenine pathway, including L-tryptophan, kynurenine, 3-hydroxykynurenine, and kynurenic acid, as well as to the aromatic amino acids (AAs) L-tyrosine and L-phenylalanine. PAEKI was performed by electrokinetic injection for 2 min at -6.5 kV (reversed polarity) and 0.5 psi (3.45 kPa) using a fused silica capillary (50 cm in length, 50 µm inner diameter). The background electrolyte (BGE) consisted of 20 mM Na

Indexed as

Amino AcidsChromatography, Micellar Electrokinetic CapillaryKynurenineHumansLimit of DetectionMicellesPressureSodium Dodecyl SulfateTryptophanAmino AcidsKynurenineMicellesSodium Dodecyl SulfateTryptophananionic compoundscapillary electrokinetic chromatographykynurenine pathwaymixed micelleon-line preconcentrationpressure-assisted electrokinetic injection

Identifiers

PMID40649903
PMCPMC12250060

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.