Methods for Mitochondrial DNA Damage and Depletion in Immortalized Trabecular Meshwork Cells.

Shane P Kennedy, Emily Tsaturian, Linlin Zhao, Joshua T Morgan

Abstract read

In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Reactivity-Guided Selective Covalent Targeting of mtDNA over nDNA by an NHC-Rhenium Complex Triggers Bioenergetic Collapse in Cancer Cells.JACS Au · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Shane P KennedyDepartment of Cell, Molecular, and Developmental Biology, University of California, Riverside, CA 92521, USA.

Emily TsaturianDepartment of Bioengineering, University of California, Riverside, CA 92521, USA.

Linlin ZhaoDepartment of Chemistry, University of California, Riverside, CA 92521, USA.ORCID 0000-0002-8821-4198

Joshua T MorganDepartment of Bioengineering, University of California, Riverside, CA 92521, USA.ORCID 0000-0001-8033-0285

Funding

Chemical and Molecular Mechanisms of Mitochondrial DNA DegradationR35GM128854 · NIGMS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI Linlin Zhao · 2018 to 2026

$3.1M

Glaucoma Foundation Grant-In-AidNIGMS NIH HHS R35 GM128854NIH HHS 5R35GM128854-25S1U.S. National Science Foundation 2046093

6 · The paper itself

Abstract

Mitochondrial DNA (mtDNA) damage in trabecular meshwork (TM) cells occurs in open-angle glaucoma (OAG). However, current in vitro models for OAG-like changes in TM cells do not explicitly incorporate mtDNA damage. This work validated two methods of mtDNA damage in immortalized TM cells and assessed OAG-associated expression changes. mtDNA was depleted in TM-1 cells via both ethidium bromide (EtBr) treatment and doxycycline (Dox) induction of a mutant (Y147A) version of Uracil DNA Glycosylase 1 (UNG1) in TM-1 cells (TM-1

Indexed as

DNA DamageDNA, MitochondrialTrabecular MeshworkCell LineDoxycyclineEthidiumGlaucoma, Open-AngleHumansMitochondriaDNA, MitochondrialDoxycyclineEthidiumglaucomamitochondriatrabecular meshwork

Identifiers

PMID40650044

DOI10.3390/ijms26136255

PMCPMC12250015

What Socratic holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.