Evidence mapPaperPMID 40650239Full record

Observational studyInternational journal of molecular sciences2025

Exploring Molecular Signatures Associated with Inflammation and Angiogenesis in the Aqueous Humor of Patients with Non-Proliferative Diabetic Retinopathy.

Víctor Alegre-Ituarte, Irene Andrés-Blasco, David Peña-Ruiz, Salvatore Di Lauro, Sara Crespo-Millas, Alessio Martucci, Jorge Vila-Arteaga, María Dolores Pinazo-Durán, David Galarreta, Julián García-Feijoo

Abstract readMulticenter StudyObservational Study
In one paragraph

Observational study in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Víctor Alegre-ItuarteCellular and Molecular Ophthalmo-Biology Group (GIUV 13/152), Department of Surgery, Faculty of Medicine and Odontology, University of Valencia, 46010 Valencia, Spain.ORCID 0000-0002-8550-4392
Irene Andrés-BlascoCellular and Molecular Ophthalmo-Biology Group (GIUV 13/152), Department of Surgery, Faculty of Medicine and Odontology, University of Valencia, 46010 Valencia, Spain.ORCID 0000-0003-2112-8217
David Peña-RuizOphthalmology Service, University Clinic Hospital, 47003 Valladolid, Spain.
Salvatore Di LauroOphthalmology Service, University Clinic Hospital, 47003 Valladolid, Spain.
Sara Crespo-MillasOphthalmology Service, University Clinic Hospital, 47003 Valladolid, Spain.
Alessio MartucciOphthalmology Service, University of Roma Tor Vergata, 00133 Roma, Italy.ORCID 0000-0002-3725-8211
Jorge Vila-ArteagaOphthalmology Service, University and Polytechnic Hospital La Fe, 46026 Valencia, Spain.
María Dolores Pinazo-DuránCellular and Molecular Ophthalmo-Biology Group (GIUV 13/152), Department of Surgery, Faculty of Medicine and Odontology, University of Valencia, 46010 Valencia, Spain.ORCID 0000-0002-0118-7837
David GalarretaOphthalmology Service, University Clinic Hospital, 47003 Valladolid, Spain.
Julián García-FeijooOphthalmology Service, University Clinic Hospital San Carlos, 28040 Madrid, Spain.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Type 2 diabetes mellitus (T2DM) is a major public health concern that significantly increases the risk of diabetic retinopathy (DR), a leading cause of visual impairment worldwide. This study aimed to identify molecular markers of inflammation (INF) and angiogenesis (ANG) in the aqueous humor (AH) of patients with non-proliferative diabetic retinopathy (NPDR). We conducted an observational, multicenter, case-control study including 116 participants classified into T2DM with NPDR, T2DM without DR, and non-diabetic controls (SCG) undergoing cataract surgery. AH samples were collected intraoperatively and analyzed for 27 cytokines using multiplex immunoassay. Eighteen immune mediators were detected in AH samples, and several were significantly elevated in the NPDR group, including the interleukins (IL) -1β, -6, -8, -15, -17, as well as the granulocyte-macrophage colony stimulating factor (GM-CSF), basic fibroblast growth factor (bFGF), interferon gamma-induced protein (IP-10), macrophage inflammatory protein 1 beta (MIP-1b), monocyte chemoattractant protein-1 (MCP-1), regulated on activation, normal T cell-expressed and -secreted protein (RANTES), and the vascular endothelial growth factor (VEGF). These molecules are involved in retinal INF, blood-retinal barrier breakdown, and pathological neovascularization. Our findings reveal a distinct pro-INF and pro-ANG profile in the AH of NPDR patients, suggesting that these cytokines may serve as early diagnostic/prognostic biomarkers for DR. Targeting these molecules could provide novel therapeutic strategies to mitigate retinal damage and vision loss in diabetic patients.

Indexed as

Aqueous HumorDiabetes Mellitus, Type 2Diabetic RetinopathyInflammationNeovascularization, PathologicAgedAngiogenesisBiomarkersCase-Control StudiesCytokinesFemaleHumansMaleMiddle AgedBiomarkersCytokinesangiogenesisaqueous humorbiomarkersinflammationmultiplex bead-based immunoassaynon-proliferative diabetic retinopathyretinatype 2 diabetes mellitus

Identifiers

PMID40650239
PMCPMC12250313

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.