Evidence map›Paper›PMID 40650988›Full record

ArticleBioinformatics (Oxford, England)2025

SCassist: an AI based workflow assistant for single-cell analysis.

Vijayaraj Nagarajan, Guangpu Shi, Samyuktha Arunkumar, Chunhong Liu, Jaanam Gopalakrishnan, Pulak R Nath, Junseok Jang, Rachel R Caspi

Abstract read
In one paragraph

Article in Bioinformatics (Oxford, England), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Vijayaraj NagarajanLaboratory of Immunology, National Eye Institute, NIH, Bethesda, MD 20892, United States.ORCID 0000-0003-2455-7172
Guangpu ShiLaboratory of Immunology, National Eye Institute, NIH, Bethesda, MD 20892, United States.
Samyuktha ArunkumarLaboratory of Immunology, National Eye Institute, NIH, Bethesda, MD 20892, United States.
Chunhong LiuNeuro-Immune Regulome Unit (Alumni), National Eye Institute, NIH, Bethesda, MD 20892, United States.
Jaanam GopalakrishnanNeuro-Immune Regulome Unit, National Eye Institute, NIH, Bethesda, MD 20892, United States.
Pulak R NathLaboratory of Immunology (Alumni), National Eye Institute, NIH, Bethesda, MD 20892, United States.
Junseok JangLaboratory of Immunology, National Eye Institute, NIH, Bethesda, MD 20892, United States.
Rachel R CaspiLaboratory of Immunology, National Eye Institute, NIH, Bethesda, MD 20892, United States.

Funding

T-CELL LINES AND CLONES IN UVEITAZ01EY000184 · NEI · NATIONAL EYE INSTITUTE · PI CASPI, RACHEL R. · 1985 to 2008
$6.6M
Understanding the microbial requirements for colonization and immunogenicity of commensal bacteria at the ocular surfaceR01EY032482 · NEI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI ST LEGER, ANTHONY J · 2021 to 2025
$2.0M
Intramural NIH HHS Z01 EY000184National Institutes of Health, National Eye Institute EY000184NEI NIH HHS R01 EY032482
6 · The paper itself

Abstract

summarySingle-cell RNA sequencing (scRNA-seq) data analysis often involves complex iterative workflow, requiring significant expertise and time. To navigate this complexity, we have developed SCassist, an R package that leverages the power of the large language models (LLM's) to guide and enhance scRNA-seq analysis. SCassist integrates LLM's into key workflow steps, to analyze user data and provide relevant recommendations for filtering, normalization and clustering parameters. It also provides LLM guided insightful interpretations of variable features and principal components, along with cell type annotations and enrichment analysis. SCassist provides intelligent assistance using popular LLM's like Google's Gemini, OpenAI's GPT and Meta's Llama3, making scRNA-seq analysis accessible to researchers at all levels. AVAILABILITY AND IMPLEMENTATION: The SCassist package, along with the detailed tutorials, is available at GitHub. https://github.com/NIH-NEI/SCassist.

Indexed as

Artificial IntelligenceSequence Analysis, RNASingle-Cell AnalysisSoftwareHumansWorkflow

Identifiers

PMID40650988
PMCPMC12341677

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.