ArticleAnalytical and bioanalytical chemistry2025
Enzyme-induced fluorescence signal-on for specific detection of alkaline phosphatase and imaging in live cells.
Article in Analytical and bioanalytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Activatable fluorescent nanosensor for real-time detection and intracellular imaging of histone deacetylase 1.Analytical and bioanalytical chemistry · 2026Article
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Authors and funding
4 authors.
Funding
Abstract
A highly sensitive and stable fluorescent strategy for detecting alkaline phosphatase (ALP) in complex samples was developed using oxidized single-walled carbon nanohorns (oxSWCNHs) and exonuclease I (Exo I). A ssDNA probe, comprising a fluorophore-labeled aptamer and a 3' phosphate group, was designed and synthesized. In the absence of ALP, the ssDNA probe binds to oxSWCNHs, causing fluorescence quenching. When ALP is present, it removes the 3' phosphate group from the ssDNA, releasing a free 3'-OH group. The dephosphorylated ssDNA is then hydrolyzed by Exo I, generating a single base and FAM, which cannot bind to oxSWCNHs, resulting in enhanced fluorescence of the system. This strategy was successfully applied to image hepatocytes, showing the potential of our sensing system for ALP bioimaging in living cells. The method has good selectivity and high sensitivity under optimized experimental conditions, with a detection limit of 0.4 mU/mL and a range of 0.5-50 mU/mL. Additionally, it was used to study the inhibitory effects of Na
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.