ArticleAdvanced healthcare materials2025
Thermoresponsive BrushGel Microcarriers for Efficient Cell Expansion and Enzyme-Reduced Harvesting.
Article in Advanced healthcare materials, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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Who cites it
4 citing papers in PubMed.
- A Hierarchical Hydrogel Modulates Immune-Mechanical Microenvironments to Spatiotemporally Reprogram Fibroblasts for Scarless Diabetic Wound Healing.Advanced healthcare materials · 2026Article
- Programmable Microcarriers for Stem Cell Therapy: Advanced Fabrication Strategies, Stem Cell Fate Regulatory Function and Biomedical Applications.International journal of molecular sciences · 2026Review
- Electroconductive Soft Microcarriers for Suspension Culture of Skeletal Muscle Cells.Macromolecular bioscience · 2026Article
- Thermoresponsive BrushGel Microcarriers for Efficient Cell Expansion and Enzyme-Reduced Harvesting.Advanced healthcare materials · 2025Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Scaling up cell therapy requires efficient expansion of high-quality cells. Microcarrier(MC)-based systems offer high surface-to-volume ratios and reduce culture media usage. In this study, we developed BrushGel, a temperature-responsive MC composed of gelatin methacryloyl (GelMA) hydrogel particles coated with poly(N-isopropyl acrylamide) (PNIPAM) polymer brushes via covalent grafting. BrushGel was fabricated using a flow-focusing droplet microfluidic device and functionalized using carbodiimide chemistry ( 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide-N-Hydroxysuccinimide, EDC-NHS). The degree of PNIPAM coating was tuned by varying the degree of methacrylation (DOM) of GelMA and the concentration of PNIPAM. Human dermal fibroblast (HNDF) cells cultured on the BrushGel under dynamic conditions showed a 4.9 fold increase in cell density, 12-fold upregulation in COL1A1 gene expression and elevated procollagen protein secretion compared to static culture. Low temperature detachment (4 °C) yeilded up to 65% detachment efficiency with >95% post-detachment viability. Clinical grade human bone marrow-derived mesenchymal stromal/stem cells (MSCs) expnaded 5.3 fold over five days on BrushGel with 69% detachment efficiency and 80% post-harvest viability using 10-fold less enzyme. BrushGel supported over 10 days of culture in spinner flasks, enabling enzyme-minimized, scalable cell expansion. These findings position BrushGel as a promising platfrom for dyanmic cell culture systems in regenerative medicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.