Evidence map›Paper›PMID 40664717›Full record

ArticleEMBO reports2025

A pivot-tether model for nucleosome recognition by the chromosomal passenger complex.

Reinis R Ruza, Chyi Wei Chung, Danny B H Gold, Michela Serena, Emile Roberts, Ulrike Gruneberg, Francis A Barr

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in EMBO reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Reinis R RuzaDepartment of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.
Chyi Wei Chung *Department of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK.
Danny B H Gold *Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.ORCID 0000-0001-6184-5494
Michela SerenaDepartment of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.
Emile RobertsDepartment of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK.ORCID 0000-0002-5729-6290
Ulrike GrunebergDepartment of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK.ORCID 0000-0001-8344-0500
Francis A BarrDepartment of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK. francis.barr@bioch.ox.ac.uk.ORCID 0000-0001-7518-253X

Funding

Cancer Research UK (CRUK) C20079/A24743Cancer Research UK (CRUK) DRCNPG-Nov21\100004Cancer Research UK (CRUK) DRCRPG-May23/100006
6 · The paper itself

Abstract

Spatial restriction of Aurora B to T3-phosphorylated histone H3 (H3pT3) nucleosomes adjacent to centromeres during prometaphase and metaphase enables it to phosphorylate proteins necessary for spindle assembly checkpoint signalling and biorientation of chromosomes on the mitotic spindle. Aurora B binding to H3pT3-nucleosomes requires a multivalent targeting module, the chromosomal passenger complex (CPC), consisting of survivin, borealin, and INCENP. To shed light on how these components mediate CPC localisation during prometaphase and metaphase, we determined the structure of the CPC targeting module in complex with haspin-phosphorylated H3pT3-nucleosomes by cryo-electron microscopy. This structure shows how the N-terminus of borealin and the survivin BIR domain act as pivot and flexible tethering points, respectively, to increase CPC affinity for H3pT3 nucleosomes without limiting it to a specific orientation. We demonstrate that this flexible, yet constrained pivot-tether arrangement is important for the control of spindle assembly checkpoint signalling by Aurora B.

Indexed as

NucleosomesAurora Kinase BCell Cycle ProteinsChromosomal Proteins, Non-HistoneCryoelectron MicroscopyHistonesHumansIntracellular Signaling Peptides and ProteinsModels, MolecularPhosphorylationProtein BindingProtein Serine-Threonine KinasesSpindle ApparatusSurvivinAURKB protein, humanAurora Kinase BBIRC5 protein, humanCDCA8 protein, humanCell Cycle ProteinsChromosomal Proteins, Non-HistoneHASPIN protein, humanHistonesINCENP protein, humanIntracellular Signaling Peptides and ProteinsNucleosomesProtein Serine-Threonine KinasesSurvivinAurora Kinase BCentromeresMitosisNucleosomesSpindle Assembly Checkpoint

Identifiers

PMID40664717
PMCPMC12420818

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.