Evidence map›Paper›PMID 40664734›Full record

ArticleScientific reports2025

Induction of cancer stem cells from mouse fibroblasts.

Snehal Shabrish, Leimarembi Devi Naorem, Shubham Mohanty, Rohit Kumar, Relestina Lopes, Nabila Akhter, Naveen Kumar Khare, Ruchi Joshi, Rajashree Sawant, Gorantla V Raghuram and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Snehal ShabrishTranslational Research Laboratory Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai, 410210, India.
Leimarembi Devi NaoremHomi Bhabha National Institute, Anushakti Nagar, Mumbai, 400094, India.
Shubham MohantyTranslational Research Laboratory Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai, 410210, India.
Rohit KumarTranslational Research Laboratory Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai, 410210, India.
Relestina LopesTranslational Research Laboratory Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai, 410210, India.
Nabila AkhterTranslational Research Laboratory Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai, 410210, India.
Naveen Kumar KhareTranslational Research Laboratory Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai, 410210, India.
Ruchi JoshiTranslational Research Laboratory Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai, 410210, India.
Rajashree SawantTranslational Research Laboratory Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai, 410210, India.
Gorantla V RaghuramTranslational Research Laboratory Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai, 410210, India.
Vishalkumar JadhavTranslational Research Laboratory Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai, 410210, India.
Sushma ShindeTranslational Research Laboratory Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai, 410210, India.
Pratik ChandraniHomi Bhabha National Institute, Anushakti Nagar, Mumbai, 400094, India. pchandrani@actrec.gov.in.
Indraneel MittraTranslational Research Laboratory Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai, 410210, India. imittra@actrec.gov.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Natural biological agents that can transform normal somatic cells into cancer stem cells, have not been identified. We earlier reported that cell free chromatin particles (cfChPs) that circulate in blood of cancer patients can horizontally transfer themselves to healthy cells to induce dsDNA breaks and inflammation. Here we show that a single cell clone D5 developed from NIH3T3 mouse fibroblast cells treated with cfChPs isolated from sera of cancer patients exhibited upregulation of stemness related transcription factors OCT4, SOX2 and KLF4 and surface markers CD34, CD44 and CD133, and the ability to form spheroids in appropriate culture medium. Transcriptome analysis revealed upregulation of the stemness-associated gene Kruppel-like factor 4 (Klf4), a key Yamanaka factor for the induction of pluripotent stem cells. Additionally, several cancer-related pathways involved in invasion, metastasis, and stemness were found to be upregulated. These pathways included those related to ERBB signaling, P53, cellular senescence, ATM, CTCF and apoptosis. Subcutaneous inoculation into SCID mice resulted in development of malignant tumours which expressed the endoderm marker alpha fetoprotein confirming their stem cell property. Our results suggest the cfChPs that circulate in blood of cancer patients can transform susceptible somatic cells into cancer stem cells with the potential to promote metastasis.

Indexed as

FibroblastsNeoplastic Stem CellsAnimalsChromatinHumansKruppel-Like Factor 4Kruppel-Like Transcription FactorsMiceMice, SCIDNIH 3T3 CellsChromatinKLF4 protein, humanKlf4 protein, mouseKruppel-Like Factor 4Kruppel-Like Transcription FactorsCancerCancer stem cellsCell-free chromatinMetastasisPluripotencyStem cells

Identifiers

PMID40664734
PMCPMC12264165

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.