Evidence mapPaperPMID 40674376Full record

ArticlePloS one2025

SOX2, PIWI proteins, and MALAT1 - plasma-based emerging biomarkers for cancer detection and monitoring.

Ekaterina Kldiashvili, Ivane Abiatari, Elene Kekelia, Saba Iordanishvili, Tornike Metreveli, Eter Dumbadze

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Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ekaterina KldiashviliPetre Shotadze Tbilisi Medical Academy, Ketevan Tsamebuli avenue 51/2, Tbilisi, Georgia.ORCID https://orcid.org/0000-0003-1764-7778
Ivane AbiatariInstitute of Medical and Public Health Research, Ilia State University, Ilia Chavchavadze Avenue 32, Tbilisi, Georgia.
Elene KekeliaPetre Shotadze Tbilisi Medical Academy, Ketevan Tsamebuli avenue 51/2, Tbilisi, Georgia.
Saba IordanishviliPetre Shotadze Tbilisi Medical Academy, Ketevan Tsamebuli avenue 51/2, Tbilisi, Georgia.
Tornike MetreveliPetre Shotadze Tbilisi Medical Academy, Ketevan Tsamebuli avenue 51/2, Tbilisi, Georgia.
Eter DumbadzePetre Shotadze Tbilisi Medical Academy, Ketevan Tsamebuli avenue 51/2, Tbilisi, Georgia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSOX2, PIWI proteins, and MALAT1 are molecular regulators implicated in cancer progression, proliferation, and epithelial-mesenchmal transition (EMT). This study evaluated their expression in plasma samples from patients with colorectal, breast, and prostate cancers, and assessed their correlations with standard immunohistochemical (IHC) markers.

methodsA total 300 participants were enrolled: 150 patients with histologically confirmed cancers (50 colorectal cancer, 50 breast cancer, and 50 prostate cancer cases) and 150 age- and sex-matched healthy controls. Plasma RNA and protein levels of SOX2, PIWIL1, PIWIL2, and MALAT1 were measured via quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. IHC scores (Ki-67, p53, E-cadherin, vimentin, estrogen receptor/progesterone receptor, human epidermal growth factor receptor 2, androgen receptor) were retrieved from clinical records. Receiver-operating characteristic curve (ROC) analysis, multivariable logistic regression (adjusting for age and sex), and Pearson's correlation coefficients were used to evaluate biomarker diagnostic performance and tumor marker associations.

resultsSOX2, PIWI proteins, and MALAT1 were significantly elevated in cancer patients versus controls (p < 0.001), with qRT-PCR and ELISA results strongly correlated. All three biomarkers showed strong positive correlations with Ki-67 (r = 0.65-0.72, p < 0.001), and MALAT1 was associated with EMT marker changes (↓E-cadherin, ↑ vimentin; p < 0.001). Adjusted ROC analysis yielded area under the curve (AUC) values of 0.82-0.89 for individual biomarkers, with sensitivity ranging from 72-84% and specificity from 75-87%. SOX2 levels showed significant correlations with Ki-67 and p53 IHC positivity in colorectal and breast cancer tissues (p < 0.01), although the functional significance of p53 staining remains inconclusive.

conclusionThe differential expression of SOX2, PIWI proteins, and MALAT1 between cancer patients and healthy controls supports their potential utility as plasma-based biomarkers for distinguishing cancer cases from non-cancer cases. These findings support their potential utility as non-invasive biomarkers for distinguishing cancer cases from healthy individuals.

Indexed as

Argonaute ProteinsBiomarkers, TumorBreast NeoplasmsColorectal NeoplasmsProstatic NeoplasmsRNA, Long NoncodingSOXB1 Transcription FactorsAdultAgedCase-Control StudiesFemaleHumansMaleMiddle AgedROC CurveArgonaute ProteinsBiomarkers, TumorMALAT1 long non-coding RNA, humanPIWIL1 protein, humanPIWIL2 protein, humanRNA, Long NoncodingSOX2 protein, humanSOXB1 Transcription Factors

Identifiers

PMID40674376
PMCPMC12270145

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.