Evidence map›Paper›PMID 40677170›Full record

ArticleMicrocirculation (New York, N.Y. : 1994)2025

Mechanically Tunable Poly(Ethylene Glycol) Diacrylate Hydrogels Reveal Stiffness-Related Impairments in Capillary Sprouting in Experimental Lung Fibrosis.

Julie Leonard-Duke, Samuel M J Agro, David J Csordas, Riley T Hannan, Anthony C Bruce, Jeffrey M Sturek, Shayn M Peirce, Lakeshia J Taite

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microcirculation (New York, N.Y. : 1994), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Julie Leonard-DukeDepartment of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.
Samuel M J AgroDepartment of Chemical Engineering, University of Virginia, Charlottesville, Virginia, USA.
David J CsordasDepartment of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.
Riley T HannanRobert M. Berne Cardiovascular Research Center, University of Virginia, Charlottesville, Virginia, USA.
Anthony C BruceDepartment of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.
Jeffrey M SturekDepartment of Pulmonary and Critical Care Medicine, University of Virginia, Charlottesville, Virginia, USA.ORCID 0000-0002-2731-3299
Shayn M PeirceDepartment of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.ORCID 0000-0001-5857-5606
Lakeshia J TaiteDepartment of Chemical Engineering, University of Virginia, Charlottesville, Virginia, USA.

Funding

Women's Oncology Program - WONP30CA044579 · NCI · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI Michael D. Solga · 1987 to 2026
$72.1M
BASIC CARDIOVASCULAR RESEARCH TRAINING GRANTT32HL007284 · NHLBI · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI Brant E Isakson, Gary K Owens · 1985 to 2026
$19.6M
Multi-scale, model-driven exploration of sub-generational gene expression in bacteria: individual consequences, population benefitsR01GM140008 · NIGMS · STANFORD UNIVERSITY · PI COVERT, MARKUS W · 2021 to 2024
$2.2M
Modeling to Design Treatments for Idiopathic Lung FibrosisR01HL155143 · NHLBI · UNIVERSITY OF VIRGINIA · PI BARKER, THOMAS HARRISON, PEIRCE-COTTLER, SHAYN · 2021 to 2024
$2.2M
Role of Natural Antibodies and B1 cells in Fibroproliferative Lung DiseaseF32HL170760 · NHLBI · UNIVERSITY OF VIRGINIA · PI HANNAN, RILEY T · 2023 to 2025
$150k
National Science Foundation NSF‐20211130 ISSNLNational Science Foundation NSF‐BMMB 2140549NCI NIH HHS P30 CA044579NHLBI NIH HHS F32 HL170760NHLBI NIH HHS R01 HL155143NHLBI NIH HHS T32 HL007284NIGMS NIH HHS R01 GM140008
6 · The paper itself

Abstract

objectiveSynthetic hydrogels that support 3D cell culture are widely used as platforms for modeling disease, such as tissue fibrosis, which leads to mechanical stiffening of the extracellular matrix (ECM). To interrogate how mechanical stiffness of the ECM affects microvascular remodeling, we developed a bioactive poly(ethylene glycol) diacrylate (PEGDA) hydrogel model with tunable stiffness that permits microvascular sprouting.

methodsLung explants harvested from healthy and fibrotic mice were cultured ex vivo on PEGDA hydrogels for 7 days. Capillary sprouting from lung segments was evaluated via imaging and secreted angiogenic markers.

resultsHealthy lung explants had decreased sprout formation and length on stiffer hydrogels. The sprouts from fibrotic lung explants, however, were not impacted by hydrogel stiffness. This difference was associated with higher expression of angiogenic markers and matrix remodeling enzymes in the fibrotic lung explants.

conclusionsOur results suggest a compensation in vasculature derived from fibrotic tissue to matrix mechanics in promoting angiogenic sprouting.

Indexed as

CapillariesHydrogelsNeovascularization, PathologicNeovascularization, PhysiologicPolyethylene GlycolsPulmonary FibrosisAnimalsExtracellular MatrixLungMiceHydrogelspoly(ethylene glycol)diacrylatePolyethylene Glycolsangiogenesisfibrosishydrogelspoly(ethylene glycol)

Identifiers

PMID40677170
PMCPMC12271678

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.