Evidence mapPaperPMID 40683252Full record

ArticleCell genomics2025

Impact of polymorphisms on gene expression and splicing in response to exercise and diet-induced weight loss in human skeletal muscle tissues.

Wenjing Wang, Wei Lin Liew, Shiqi Huang, Edmund Chan, Amelia Li Min Tan, Chi Tian, Yihan Tong, Yuntian Zhang, Fei Liu, Yixian Qin and 17 more

Abstract read
In one paragraph

Article in Cell genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

27 authors.

Wenjing WangDepartment of Pharmacy and Pharmaceutical Sciences, Faculty of Science, National University of Singapore, Singapore 117559, Singapore.
Wei Lin LiewDepartment of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.
Shiqi HuangDepartment of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.
Edmund ChanDepartment of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.
Amelia Li Min TanDepartment of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore; Duke-National University of Singapore Medical School, Singapore 169857, Singapore.
Chi TianDepartment of Pharmacy and Pharmaceutical Sciences, Faculty of Science, National University of Singapore, Singapore 117559, Singapore.
Yihan TongDepartment of Pharmacy and Pharmaceutical Sciences, Faculty of Science, National University of Singapore, Singapore 117559, Singapore.
Yuntian ZhangDepartment of Biomedical Informatics, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.
Fei LiuDepartment of Pharmacy and Pharmaceutical Sciences, Faculty of Science, National University of Singapore, Singapore 117559, Singapore.
Yixian QinDepartment of Pharmacy and Pharmaceutical Sciences, Faculty of Science, National University of Singapore, Singapore 117559, Singapore.
Sean Jun Leong OuDepartment of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore; Department of Food Science and Technology, National University of Singapore, Singapore 117543, Singapore.
Suresh Anand SadananthanInstitute for Human Development and Potential (IHDP), Agency for Science, Technology and Research (A∗STAR), Singapore 117609, Singapore.
Sambasivam Sendhil VelanInstitute for Human Development and Potential (IHDP), Agency for Science, Technology and Research (A∗STAR), Singapore 117609, Singapore; Human Magnetic Resonance Center (hMRC), Institute for Applied Life Sciences, University of Massachusetts, Amherst, Amherst, MA 01003, USA.
Kavita VenkataramanSaw Swee Hock School of Public Health, National University of Singapore and National University Health System, Singapore 117549, Singapore.
Sarah R LangleyDuke-National University of Singapore Medical School, Singapore 169857, Singapore; School of Bioscience, Cardiff University, Cardiff CF10 3AX, UK.
Petretto EnricoDuke-National University of Singapore Medical School, Singapore 169857, Singapore.
Shawn HoonMolecular Engineering Laboratory, IMCB, Agency for Science, Technology and Research (A∗STAR), Singapore 138673, Singapore.
Kwang Wei ThamDepartment of Endocrinology, Woodlands Health, National Healthcare Group, Singapore 737628, Singapore.
Yap Seng ChongInstitute for Human Development and Potential (IHDP), Agency for Science, Technology and Research (A∗STAR), Singapore 117609, Singapore; Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.
Yung Seng LeeInstitute for Human Development and Potential (IHDP), Agency for Science, Technology and Research (A∗STAR), Singapore 117609, Singapore; Department of Paediatrics, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.
Melvin Khee-Shing LeowInstitute for Human Development and Potential (IHDP), Agency for Science, Technology and Research (A∗STAR), Singapore 117609, Singapore; Duke-National University of Singapore Medical School, Singapore 169857, Singapore.
Xueling SimSaw Swee Hock School of Public Health, National University of Singapore and National University Health System, Singapore 117549, Singapore.
Chin Meng KhooDepartment of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore; Division of Endocrinology, Department of Medicine, National University Health System, Singapore 119228, Singapore.
E Shyong TaiDepartment of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore; Duke-National University of Singapore Medical School, Singapore 169857, Singapore; Division of Endocrinology, Department of Medicine, National University Health System, Singapore 119228, Singapore.
Eric Yin Hao KhooDepartment of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.
Mei Hui LiuDepartment of Food Science and Technology, National University of Singapore, Singapore 117543, Singapore. Electronic address: fstlmh@nus.edu.sg.
Boxiang LiuDepartment of Pharmacy and Pharmaceutical Sciences, Faculty of Science, National University of Singapore, Singapore 117559, Singapore; Department of Biomedical Informatics, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore; Genome Institute of Singapore (GIS), Agency for Science, Technology and Research (A∗STAR), Singapore 138672, Singapore; Precision Medicine Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117596, Singapore; NUS Centre for Cancer Research, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117599, Singapore; Cardiovascular-Metabolic Disease Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117599, Singapore. Electronic address: boxiangliu@nus.edu.sg.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Weight loss through exercise and diet reduces the risk of type 2 diabetes, but the genetic regulation of gene expression and splicing in response to weight loss remains unclear in humans. We collected clinical data and skeletal muscle biopsies from 54 overweight/obese Asian individuals before and after a 16-week lifestyle intervention, which resulted in an average of ∼10% weight loss, accompanied by an ∼30% increase in insulin-stimulated glucose uptake. Improvements were observed in 118 of 252 clinical traits and six blood lipids. Transcriptomic analysis of paired skeletal muscle biopsies identified 505 differentially expressed genes enriched in mitochondrial function and insulin sensitivity. Thousands of muscle-specific expression/splicing quantitative trait loci (e/sQTLs) were detected pre- and post-intervention, including hundreds of lifestyle-responsive e/sQTLs. Notably, approximately 4.2% of eQTLs and 7.3% of sQTLs showed Asian specificity. Joint analysis with genome-wide association study (GWAS) identified 16 putative metabolic risk genes. Our study reveals gene-by-lifestyle interactions and how lifestyle modulates gene regulation in skeletal muscle.

Indexed as

DietExerciseMuscle, SkeletalPolymorphism, Single NucleotideWeight LossAdultFemaleGene Expression RegulationGenome-Wide Association StudyHumansInsulin ResistanceMaleMiddle AgedObesityQuantitative Trait LociAsian-specific QTLgene-by-lifestyle interactionhuman intervention studylifestyle modificationsmetabolic diseaseskeletal muscle transcriptomicsweight loss

Identifiers

PMID40683252
PMCPMC12534703

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.