Evidence mapPaperPMID 40684027Full record

ArticleInternational journal of obesity (2005)2025

Single-cell transcriptome atlas and genome-wide Mendelian randomization reveal chemokine involvement in diverse immune cells in type 2 diabetes.

Yang Liu, Tao Wang, Rong Wu, Jiapeng Gao, Jiexun Cai, Lixia Huo, Xiaoyu Li, Jingwen Li, Jue Wang, Zhen Wang and 2 more

Abstract read
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Article in International journal of obesity (2005), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Yang LiuHuzhou Key Laboratory of Chronic Kidney Disease, First Affiliated Hospital, Huzhou University, Huzhou, China.
Tao WangSchool of Medicine & Nursing, Huzhou University, Huzhou, China.
Rong WuHuzhou Key Laboratory of Chronic Kidney Disease, First Affiliated Hospital, Huzhou University, Huzhou, China.
Jiapeng GaoSchool of Medicine & Nursing, Huzhou University, Huzhou, China.
Jiexun CaiSchool of Medicine & Nursing, Huzhou University, Huzhou, China.
Lixia HuoHuzhou Key Laboratory of Chronic Kidney Disease, First Affiliated Hospital, Huzhou University, Huzhou, China.
Xiaoyu LiSchool of Medicine & Nursing, Huzhou University, Huzhou, China.
Jingwen LiSchool of Medicine & Nursing, Huzhou University, Huzhou, China.
Jue WangSchool of Medicine & Nursing, Huzhou University, Huzhou, China.
Zhen WangSchool of Medicine & Nursing, Huzhou University, Huzhou, China.
Xiaoyi WangHuzhou Key Laboratory of Chronic Kidney Disease, First Affiliated Hospital, Huzhou University, Huzhou, China.
Yunliang YaoHuzhou Key Laboratory of Chronic Kidney Disease, First Affiliated Hospital, Huzhou University, Huzhou, China. yaoyunliang@zjhu.edu.cn.ORCID http://orcid.org/0000-0002-6864-2021

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundChemokine-driven immune dysregulation is increasingly recognized as a hallmark of T2D pathogenesis(T2D), where insulin resistance and metabolic stressors drive chronic inflammation. While chemokine cascades are hypothesized to mediate diabetic immunopathology, causal mediators remain undefined.

methodsWe employed Mendelian Randomization (MR) of genome-wide association studies to identify causal inflammatory mediators, serological validation in streptozotocin-induced murine T2D models, and single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells (PBMC) to map immune cell heterogeneity and intercellular communication networks.

resultsMR prioritized IFN-γ, CCL7, MIF, and CXCL9 as genetically supported T2D effectors. Murine validation confirmed CCL7 and MIF as robust circulating mediators. scRNA-seq revealed compartment-specific chemokine receptor dynamics (CCR4/5/6, CXCR3/4/5, CX3CR1), dominated by enhanced CCL5-CCR5 and CCL6-CCR2 crosstalk.

conclusionThis work establishes a systems-level framework for chemokine signaling in T2D immunopathogenesis, identifying nodal regulators of immune crosstalk as potential therapeutic vulnerabilities.

Indexed as

ChemokinesDiabetes Mellitus, Type 2TranscriptomeAnimalsGenome-Wide Association StudyHumansLeukocytes, MononuclearMaleMendelian Randomization AnalysisMiceSingle-Cell AnalysisChemokines

Identifiers

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.