Evidence map›Paper›PMID 40694471›Full record

ArticleSTAR protocols2025

Protocol for assembling photostable fluorescence-labeled tiny DNA origami blocks to observe kinesin cooperation.

Tomoki Kita, Ryota Sugie, Yuki Suzuki, Shinsuke Niwa

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Tomoki KitaGraduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai, Miyagi 980-8577, Japan.
Ryota SugieDepartment of Applied Chemistry, Graduate School of Engineering, Mie University, 1577 Kurimamachiya-cho, Tsu, Mie 514-8507, Japan.
Yuki SuzukiDepartment of Applied Chemistry, Graduate School of Engineering, Mie University, 1577 Kurimamachiya-cho, Tsu, Mie 514-8507, Japan. Electronic address: ysuzuki@chem.mie-u.ac.jp.
Shinsuke NiwaGraduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai, Miyagi 980-8577, Japan; Frontier Research Institute for Interdisciplinary Sciences (FRIS), Tohoku University, Aramaki-Aoba 6-3, Aoba-ku, Sendai, Miyagi 980-0845, Japan. Electronic address: shinsuke.niwa.c8@tohoku.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fluorescence-labeled tiny DNA origami blocks (FTOBs) enable observation of kinesin motility and their cooperation with minimal blinking and photobleaching. Here, we present a protocol for assembling FTOBs to observe kinesin cooperation. We describe steps for linking FTOBs and kinesins via a rationally designed epitope tag (ALFA-tag) and its high-affinity single-domain nanobody (NbALFA). We detail procedures for connecting two FTOB-kinesin complexes via hybridization of complementary DNA arms and their single-molecule tracking. This protocol has potential applications in any motor proteins. For complete details on the use and execution of this protocol, please refer to Kita et al.

Indexed as

DNAFluorescent DyesKinesinsHumansSingle Molecule ImagingDNAFluorescent DyesKinesinsBiophysicsBiotechnology and bioengineeringMicroscopyMolecular BiologyMolecular/Chemical ProbesProtein BiochemistryProtein expression and purificationSingle-molecule Assays

Identifiers

PMID40694471
PMCPMC12491155

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.