ArticleScientific reports2025
Identification of SnRNA U6 as an endogenous reference gene for normalization of MiRNA expression data in COVID-19 patients.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- Identification of stable miRNA references for qRT-PCR normalization in ovine fasciolosis.BMC genomics · 2026Article
- Validation of Guanidine-EDTA as a Preservative Agent for the Analysis of miRNAs and mRNAs in Blood Samples of Chagas Disease Patients.Pathogens (Basel, Switzerland) · 2026Article
- Role of immune-inflammatory biomarkers and their derived ratio in predicting COVID-19 severity and mortality.Scientific reports · 2025Article
- Sustained Higher Levels of Plasma hsa-miR-17-5p Expression During Gestational Diabetes Mellitus and Postpartum.Epigenomes · 2025Article
- MiRNA sequencing of platelet and exosome revealed platelet miR-199b-3p as a potential biomarker in lung adenocarcinoma.Frontiers in immunology · 2025Article
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Authors and funding
11 authors.
Funding
Abstract
SARS-CoV-2, the virus responsible for COVID-19, exhibits structural differences compared to other coronaviruses that impact transmission dynamics and host response. Although vaccines have reduced severe hospitalizations and deaths, understanding the infection remains challenging, and finding viable biomarkers for disease severity would be beneficial. In this context, microRNAs (miRNAs) are a set of small non-coding RNAs that have emerged as potential biomarkers for numerous diseases. However, assessing miRNA expression requires the normalization of RT-qPCR data using appropriate endogenous reference genes. The selection of these reference genes remains a major challenge, directly impacting the accuracy of expression analyses. Although several small RNAs, including snRNAs, have been evaluated, there is still no consensus regarding the optimal reference genes for the normalization of plasma miRNA expression data in COVID-19 infection. This study evaluated five candidate genes-including RNU6B, snRNA U6 (small nuclear RNAs) and three miRNAs (miR-320a, miR-342-3p, and miR-328) as potential endogenous normalizers for analyzing miRNA expression in the plasma of COVID-19 patients. The snRNA U6 showed greater stability using a combination of statistical algorithms (NormFinder, RefFinder, BestKeeper, and GeNorm), indicating that this snRNA can be used as a robust internal reference for analysis in the plasma of COVID-19 patients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.