Evidence map›Paper›PMID 40699432›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Inhibition of Nonsense-Mediated Decay in Dorsal Root Ganglion Neurons.

Maddy R Koch, Jenna B Demeter, Rebecca Chase, Nikesh P Kunder, Tzu-Fang Lou, Tarjani N Shukla, June Bryan I de la Peña

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Maddy R KochDepartment of Anesthesiology and Critical Care Medicine, School of Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.ORCID https://orcid.org/0009-0003-9700-1578
Jenna B DemeterDepartment of Anesthesiology and Critical Care Medicine, School of Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.ORCID https://orcid.org/0000-0001-5831-9442
Rebecca ChaseDepartment of Genetics and Department of Psychiatry, Washington University School of Medicine, St. Louis, MO, USA.ORCID https://orcid.org/0000-0001-8178-289X
Nikesh P KunderDepartment of Laboratory Medicine, University of Texas at M.D. Anderson Cancer Center, Houston, TX, USA.ORCID https://orcid.org/0000-0002-8456-7703
Tzu-Fang LouOffice of Research and Innovation, University of Texas at Dallas, Richardson, TX, USA.
Tarjani N ShuklaScience and Technology, 59th Medical Wing (59 MDW)/Chief Scientist's Office, Defense Health Agency, JBSA-Lackland AFB, San Antonio, TX, USA.
June Bryan I de la PeñaDepartment of Anesthesiology and Critical Care Medicine, School of Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, USA. jdelapena@salud.unm.edu.ORCID https://orcid.org/0000-0001-9391-7595

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Given the unique morphology of neurons with extensive projections, such as nociceptors, mRNA regulation is crucial for their physiological processes and function. Tools for manipulating post-transcriptional and translational regulatory pathways in nociceptors are essential to study the role of these pathways in pain. Nonsense-mediated decay (NMD) is a key quality-control and regulatory mechanism in which, canonically, mRNA with premature termination codons are targeted and degraded to protect the cell against aberrant truncated proteins. Inhibition of NMD has been shown to lead to nociceptive sensitization in dorsal root ganglion (DRG) sensory neurons, suggesting the relevance of NMD to pain chronification. Here we describe a method to inhibit NMD in cultured murine DRG neurons and analyze NMD-sensitive transcripts.

Indexed as

Ganglia, SpinalNeuronsNonsense Mediated mRNA DecayAnimalsCells, CulturedMiceRNA, MessengerRNA, Messenger11jActinomycin D (ActD)Chronic painNociceptionPost-transcriptional regulationRNA-seqSMG1

Identifiers

PMID40699432

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.