Evidence map›Paper›PMID 40700270›Full record

ReviewProteomes2025

Next-Generation Protein-Ligand Interaction Networks: APEX as a Powerful Technology.

José Miguel Quintero-Ferrer, Lucas Silva de Oliveira, Paula Marian Vieira Goulart, Thiago Albuquerque Souza Campos, Coralie Martin, Philippe Grellier, Izabela Marques Dourado Bastos, Sébastien Charneau

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Proteomes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

José Miguel Quintero-FerrerLaboratory of Biochemistry and Protein Chemistry, Department of Cell Biology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil.ORCID 0000-0002-1488-7947
Lucas Silva de OliveiraLaboratory of Biochemistry and Protein Chemistry, Department of Cell Biology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil.
Paula Marian Vieira GoulartLaboratory of Biochemistry and Protein Chemistry, Department of Cell Biology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil.ORCID 0000-0001-9330-7447
Thiago Albuquerque Souza CamposLaboratory of Biochemistry and Protein Chemistry, Department of Cell Biology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil.ORCID 0000-0002-5640-6478
Coralie MartinUMR 7245 MCAM Molecules of Communication and Adaptation of Microorganisms, Muséum National d'Histoire Naturelle, CNRS, 75231 Cedex 05 Paris, France.
Philippe GrellierUMR 7245 MCAM Molecules of Communication and Adaptation of Microorganisms, Muséum National d'Histoire Naturelle, CNRS, 75231 Cedex 05 Paris, France.
Izabela Marques Dourado BastosPathogen-Host Interface Laboratory, Department of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasilia 70910-900, Brazil.ORCID 0000-0002-2785-4255
Sébastien CharneauLaboratory of Biochemistry and Protein Chemistry, Department of Cell Biology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, Brazil.ORCID 0000-0002-9364-7289

Funding

Coordenação de Aperfeicoamento de Pessoal de Nível Superior COFECUB programme [88881.711954/2022-01]Coordenação de Aperfeicoamento de Pessoal de Nível Superior PROEX programmeFoundation for Research Support of the Federal District [FAPDF-00193-00000779/2021-40]National Council for Scientific and Technological Development [313942/2021-8; 316892/2023-8]
6 · The paper itself

Abstract

Peroxidases are essential enzymes that catalyze redox reactions, with wide-ranging biological implications. Among these, an enhanced ascorbate peroxidase (APEX) has emerged as a valuable tool for studying intricate intracellular events with spatiotemporal precision, particularly in protein-protein, protein-RNA, and protein-DNA interaction networks in living cells. This review discusses APEX's structural and functional attributes, its evolution through genetic engineering, and its transformative applications in high-resolution mapping used for proteomic and transcriptomic studies. Furthermore, it highlights recent advancements in substrate innovation and addresses current challenges and future directions in leveraging APEX for cutting-edge biological research.

Indexed as

engineered ascorbate peroxidasemass spectrometrymolecular networksprotein interactomesprotein–protein interactionsproximity labeling

Identifiers

PMID40700270
PMCPMC12286260

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.