ArticleScientific reports2025
Construction of dynamic ceRNA regulatory networks in osteogenesis during fracture healing based on transcriptomic analysis.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Upregulation of lncRNA SSTR5-AS1 promotes osteoblast differentiation and reduces apoptosis.Journal of orthopaedic surgery and research · 2025Article
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Authors and funding
9 authors.
Funding
Abstract
Fracture healing is a complex biological process. This study aimed to investigate the key molecules involved in fracture healing and their potential competing endogenous RNA (ceRNA) regulatory mechanisms within the first 28 days post-fracture using bioinformatics methods. The experiment was conducted on 15 adult male SD rats, with tibia callus tissue samples collected at days 0, 3, 7, 14, and 28 (n = 3) post-fracture. RNA-Seq was used for high-throughput transcriptome sequencing, followed by differential expression analysis to identify differentially expressed genes (DEGs), long non-coding RNA (DELs), and microRNA (DEMs) at different stages. Protein-protein interaction (PPI) networks were constructed using the STRING database and visualized with Cytoscape. GO and KEGG enrichment analyses were performed to explore potential biological mechanisms. miRNA-mRNA interactions were predicted using TargetScan, miRWalk, and miRDB, while RNA22 v2 was used for lncRNA-miRNA interactions. These interactions were integrated into ceRNA networks. Finally, qRT-PCR validated key molecules within the ceRNA network. We identified 4,997 DEGs, 315 DELs, and 89 DEMs at day 3; 5,087 DEGs, 300 DELs, and 84 DEMs at day 7; 3,073 DEGs, 235 DELs, and 68 DEMs at day 14; and 2,609 DEGs, 197 DELs, and 90 DEMs at day 28. Further analysis revealed hub osteogenic genes and their ceRNA regulatory networks at each time point. The networks consisted of 2 mRNAs, 3 miRNAs, and 9 lncRNAs at day 3; 2 mRNAs, 3 miRNAs, and 8 lncRNAs at days 7 and 14; and 1 mRNA, 3 miRNAs, and 10 lncRNAs at day 28. We validated two key lncRNAs (AABR07030366.1 and AABR07057997.1) along with their interacting miRNAs and mRNAs: rno-miR-9a-5p/Col9a1 (day 3), rno-miR-181c-5p/Comp (day 7), rno-miR-423-5p/Col1a1 (day 14), and rno-miR-185-5p/Ctsk (day 28). In summary, our study leveraged bioinformatics to construct ceRNA networks involved in osteogenesis post-fracture, offering insights into their dynamic regulatory role in healing and underlying molecular mechanisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.