Evidence map›Paper›PMID 40708260›Full record

ArticleElectrophoresis2025

Label-Free Sorting of Human Mesenchymal Stem Cells Using Insulating Dielectrophoresis.

Zuri A Rashad, Kiara L Lacy, Emmanuel Egun, Jazmine S Moore, Tayloria N G Adams

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Electrophoresis, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Zuri A RashadDepartment of Chemical and Biomolecular Engineering, Samueli School of Engineering, University of California, Irvine, California, USA.
Kiara L LacyDepartment of Chemical and Biomolecular Engineering, Samueli School of Engineering, University of California, Irvine, California, USA.
Emmanuel EgunDepartment of Chemical and Biomolecular Engineering, Samueli School of Engineering, University of California, Irvine, California, USA.
Jazmine S MooreSue & Bill Gross Stem Cell Research Center, University of California, Irvine, California, USA.
Tayloria N G AdamsDepartment of Chemical and Biomolecular Engineering, Samueli School of Engineering, University of California, Irvine, California, USA.

Funding

Training Program in Stem Cell Translational Medicine for Neurological DisordersT32NS082174 · NINDS · UNIVERSITY OF CALIFORNIA-IRVINE · PI PETER John DONOVAN, Leslie Michels Thompson · 2013 to 2026
$3.3M
Optically Promoting Cardiac Maturation Using Engineered PeptidesR01HL164348 · NHLBI · UNIVERSITY OF CALIFORNIA-IRVINE · PI Herdeline Ann Mallari Ardona · 2023 to 2026
$2.3M
National Institute of Health, National Heart, Lung, and Blood Institute 3R01HL164348-01A1S1National Institute of Health, National Institute of Neurological Disorders and Stroke NS082174National Science Foundation 2048221NHLBI NIH HHS R01 HL164348NINDS NIH HHS T32 NS082174Office of the President, University of California
6 · The paper itself

Abstract

Human mesenchymal stem cells (hMSCs) are a multipotent yet heterogeneous cell population with immunosuppressive and regenerative properties, making them highly promising for stem cell therapies targeting metabolic diseases. However, the inherent heterogeneity of hMSCs presents challenges for producing consistent therapeutic outcomes, emphasizing the need to isolate functionally distinct subpopulations. In this study, we employed insulating dielectrophoresis (DEP) via a trap-and-release sorting strategy to generate and characterize subpopulations of adipose tissue (AT)-derived hMSCs. Voltage and frequency parameters were systematically tuned, revealing that higher voltages increased the percentage of trapped cells, while higher frequencies had less impact. Sorted cells underwent a 14-day adipogenic differentiation process, assessed by Oil Red O staining. Our results demonstrated that untrapped cell populations generated at lower voltage and frequency thresholds exhibited enhanced adipogenic differentiation compared to unsorted controls. These findings suggest that DEP can be leveraged to isolate progenitor cells within hMSC populations, enabling the production of homogeneous cell subsets with targeted functional potential. This work highlights the utility of insulating DEP for addressing hMSC heterogeneity and advancing the development of stem cell therapies.

Indexed as

Cell SeparationElectrophoresisMesenchymal Stem CellsAdipogenesisAdipose TissueCell DifferentiationCells, CulturedHumans

Identifiers

PMID40708260
PMCPMC12532083

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.