Evidence mapPaperPMID 40708749Full record

ArticleFrontiers in cellular and infection microbiology2025

Immune and hematologicak responses to the third dose of an mRNA COVID-19 vaccine: a six-month longitudinal study.

Waleed M Bawazir, Ahmad Al Ibad, Muneeba Mohsin, Hanouf A Niyazi, Turki A Alamri, Mohammed A Bazuhair, Mohannad Hazzazi, Noura A Chehab, Steve Harakeh, Yasar Mehmood Yousafzai

Abstract read
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Waleed M Bawazir *Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
Ahmad Al Ibad *Institute of Pathology and Diagnostic Medicine, Khyber Medical University, Peshawar, Pakistan.
Muneeba MohsinDepartment of Biochemistry, Institute of Chemical and Life Sciences, Abdul Wali Khan University, Mardan, Pakistan.
Hanouf A NiyaziDepartment of Clinical Microbiology and Immunology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
Turki A AlamriFamily and Community Medicine Department, Faculty of Medicine in Rabigh, King Abdulaziz University, Jeddah, Saudi Arabia.
Mohammed A BazuhairCenter of Excellence for Drug Research and Pharmaceutical Industries, King Abdulaziz University, Jeddah, Saudi Arabia.
Mohannad HazzaziDepartment of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
Noura A ChehabNEOM Energy & Water Company (ENOWA), Neom, Saudi Arabia.
Steve HarakehEcoHealth Unit, 14 King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
Yasar Mehmood YousafzaiInstitute of Pathology and Diagnostic Medicine, Khyber Medical University, Peshawar, Pakistan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The deployment of mRNA vaccines against SARS-CoV-2 is a major landmark in controlling the COVID-19 pandemic. However, the activation of adaptive immunity and its longevity after a booster dose warrant further investigation. Moreover, the interplay between inflammation and immune thrombosis after transfection needs further insights that could help examine the vaccine's potential for adverse events following immunization (AEFIs). This study investigates the biochemical and hematological responses to the third dose of a COVID-19 mRNA vaccine in 68 healthy participants who had previously received two doses of the vaccine. Blood samples were collected at baseline (before vaccine dose; D0), 48 hours post-vaccination (D2), and then at days 30, 60, 120, and 180 (D30, D60, D120, D180). The study focused on analyzing changes in anti-SARS-COV-2 immunoglobulins (IgG and IgA), inflammatory biomarkers (IL-6, IFN-γ, CRP, hs-CRP), coagulation factors (PT, APTT, D-dimers), and blood cell counts (neutrophils, leukocytes, platelets) at D2 post-vaccination, and IgG and IgA at days 2, 30, 60, 120, and 180 post-vaccination. In this study, no clinical AEFIs were observed in any of the recipients. Slight changes were observed in the levels of inflammatory and coagulation biomarkers, and blood cells. Levels of CRP and hs-CRP increased slightly but significantly, d-dimers were raised, and PT and aPTT were prolonged significantly. A small but significant decrease was observed in IFN-γ and mean lymphocyte counts, whereas no change was observed in the levels of IL-6, neutrophils, and platelet count at D2. Levels of IgG and IgA showed sustained increase over the six-month period. These results collectively demonstrate that the third dose of the mRNA vaccine elicits a rapid and sustained immune response characterized by increased IgG and IgA levels. The changes observed in inflammatory markers and coagulation factors after vaccination observed shortly after vaccination require further investigations.

Indexed as

COVID-19COVID-19 VaccinesSARS-CoV-2AdultAntibodies, ViralBiomarkersBlood Cell CountBNT162 VaccineFemaleHumansImmunization, SecondaryImmunoglobulin AImmunoglobulin GLongitudinal StudiesMaleMiddle AgedAntibodies, ViralBiomarkersBNT162 VaccineCOVID-19 VaccinesImmunoglobulin AImmunoglobulin GmRNA VaccinesVaccines, SyntheticAEFIscoagulation profilecomplete blood countsCOVID-19 mRNA vaccineIgAIgGinflammatory cytokines

Identifiers

PMID40708749
PMCPMC12286925

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.