Evidence map›Paper›PMID 40712541›Full record

ArticleThe Science of the total environment2025

Long term assessment of SARS-CoV-2 in wastewater and the transition to evaluate additional viral targets.

Ayaaz Amirali, Mark E Sharkey, Shruti Choudhary, Kristina M Babler, Cynthia C Beaver, Pratim Biswas, Kate R Bowie, Taylor Burke, Benjamin B Currall, George S Grills and 16 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Science of the total environment, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

26 authors.

Ayaaz AmiraliDepartment of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL 33146, USA.
Mark E SharkeyDepartment of Medicine, University of Miami Miller School of Medicine, Miami 33136, FL, USA.
Shruti ChoudharyDepartment of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL 33146, USA.
Kristina M BablerDepartment of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL 33146, USA; Currently with the Department of Human Genetics, University of Utah, Salt Lake City, UT 84112, USA.
Cynthia C BeaverSylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Pratim BiswasDepartment of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL 33146, USA.
Kate R BowieDepartment of Chemical and Environmental Engineering, Yale University, New Haven, CT 06511, USA.
Taylor BurkeDepartment of Chemical and Environmental Engineering, Yale University, New Haven, CT 06511, USA.
Benjamin B CurrallSylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
George S GrillsSylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Hannah G HealyDepartment of Environmental Health, T.H. Chan School of Public Health, Harvard University, Boston, MA 02115, USA.
Alexander G LucaciDepartment of Physiology and Biophysics, Weill Cornell Medical College, New York City, NY 10021, USA; The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY 10021, USA.
Christopher E MasonDepartment of Physiology and Biophysics, Weill Cornell Medical College, New York City, NY 10021, USA; The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY 10021, USA; The WorldQuant Initiative for Quantitative Prediction, Weill Cornell Medicine, New York, NY 10021, USA.
Michaela McGuireFlorida Department of Health, Tallahassee, FL 32399, USA.
Rosemarie RamosFlorida Department of Health, Tallahassee, FL 32399, USA.
Madelena RuedafloresDepartment of Chemical and Environmental Engineering, Yale University, New Haven, CT 06511, USA.
Natasha Schaefer SolleDepartment of Medicine, University of Miami Miller School of Medicine, Miami 33136, FL, USA; Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Stephan C SchürerSylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA; Department of Molecular & Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, FL 33136, USA; Institute for Data Science & Computing, University of Miami, Coral Gables, FL 33146, USA.
Bhavarth S ShuklaDepartment of Medicine, University of Miami Miller School of Medicine, Miami 33136, FL, USA.
Mario StevensonDepartment of Medicine, University of Miami Miller School of Medicine, Miami 33136, FL, USA.
Dušica VidovićSylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA; Department of Molecular & Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Sion L WilliamsSylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Kongyang ZhuDepartment of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL 33146, USA; Department of Industrial and Systems Engineering, University of Miami, Coral Gables, FL 33146, USA.
Alessandro ZulliDepartment of Chemical and Environmental Engineering, Yale University, New Haven, CT 06511, USA; Department of Civil and Environmental Engineering, Stanford University, Stanford, CA 94301, USA.
Jordan PecciaDepartment of Chemical and Environmental Engineering, Yale University, New Haven, CT 06511, USA. Electronic address: jordan.peccia@yale.edu.
Helena M Solo-GabrieleDepartment of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL 33146, USA. Electronic address: hmsolo@miami.edu.

Funding

University of Miami Developmental Center for AIDS Research (D-CFAR)P30AI073961 · NIAID · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI Savita Pahwa · 2007 to 2026
$33.8M
Tumor Biology Research ProgramP30CA240139 · NCI · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI Stephen D. Nimer · 2019 to 2026
$24.1M
MEDICAL INFORMATICS RESEARCH TRAINING AT YALET15LM007056 · NLM · YALE UNIVERSITY · PI Mark Bender Gerstein, LUCILA OHNO-MACHADO · 1987 to 2026
$22.2M
Supplement for MINI point-of-use deviceU01DA053941 · NIDA · UNIVERSITY OF MIAMI CORAL GABLES · PI MASON, CHRISTOPHER EDWARD, SCHURER, STEPHAN C · 2021 to 2022
$5.2M
NCI NIH HHS P30 CA240139NIAID NIH HHS P30 AI073961NIDA NIH HHS U01 DA053941NLM NIH HHS T15 LM007056
6 · The paper itself

Abstract

The COVID-19 pandemic caused by the SARS-CoV-2 virus dramatically impacted society over five years ago and continues to have an impact today. Since the beginning of the pandemic there have responses and strategies implemented to maintain the public safety of communities affected by SARS-CoV-2. This study is a unique opportunity to analyze nearly four years of SARS-CoV-2 wastewater-based surveillance (WBS) data, obtained from five different laboratories, combined with four years of human health data from three adjacent regions of a large urban community (Miami-Dade County). The objective of this study was to analyze that data and evaluate longitudinal and geographic trends of SARS-CoV-2 levels in wastewater (WW) during the extensive time frame of this study. Additionally, WBS data were analyzed for multiple targets (influenza A/B, norovirus, RSV, HMPV, PMMoV) other than SARS-CoV-2 to assess the potential for expanding WBS to a wider range of targets. We found that SARS-CoV-2 levels correlated strongest with clinical positivity rates across all three geographic regions (Spearman r = 0.81 for the entire period of record), with the most geographically restricted region showing higher correlations (South, r = 0.86) than the region with populations with higher geographic mobility (North, r = 0.69). Stronger correlations (0.80 < r < 0.97) were observed when correlations were established by variant waves rather than single or multiple year time frames (0.73 < r < 0.88). When analyzing the data for targets beyond SARS-CoV-2, results show promise as two laboratories detected norovirus, influenza A/B, RSV, and HMPV at statistically not different frequencies (Chi-squared≥0.6). Overall, results suggest that the clinical metrics used (e.g., positivity), geography, and the time frames of data analyses influence the ability of WBS to predict disease prevalence in a community. The consistency among the laboratories supports that the measurement of a wider range of viral targets can be disaggregated among different laboratories providing flexibility for building national-level WBS programs.

Indexed as

COVID-19Environmental MonitoringSARS-CoV-2WastewaterHumansWastewaterClinical testingCOVID-19Influenza a/BRSVSARS-CoV-2Wastewater

Identifiers

PMID40712541
PMCPMC12490206

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.