Evidence map›Paper›PMID 40712808›Full record

ArticleJournal of advanced research2026

Efficient derivation of stable sheep embryonic stem cells opens a new avenue for agricultural and biomedical application.

Miaohan Jin, Shuhong Huang, Shiwei Zhou, Wenwen Shen, Jing Cao, Shengjiao Song, Yinghui Wei, Peter Kalds, Jinlian Hua, Baohua Ma and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of advanced research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Miaohan JinKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Shuhong HuangKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Shiwei ZhouCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
Wenwen ShenKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Jing CaoKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China; Institute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Chinese Academy of Sciences, Shanghai 200031, China.
Shengjiao SongKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Yinghui WeiKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Peter KaldsYazhouwan National Laboratory, Sanya 572024, China.
Jinlian HuaCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
Baohua MaCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
Pablo Juan RossDepartment of Animal Science, University of California, Davis CA 95616, USA.
Xiaolong WangKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. Electronic address: xiaolongwang@nwafu.edu.cn.
Yulin ChenKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. Electronic address: chenyulin@nwafu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionEmbryonic stem cells (ESCs), capable of generating all adult cell types, hold transformative potential for agriculture and biomedicine. However, stable sheep ESCs derivation remains challenging, limiting their application in further research.

objectivesWe aimed to establish stable sheep ESCs using a simplified protocol, validate their pluripotency, and demonstrate genome editing utility.

methodsSheep ESCs were derived from blastocyst inner cell masses via an IWR-1-containing culture system (termed TePR). Pluripotency was assessed through long-term culture (>100 passages), trilineage differentiation, transcriptomic analysis (E0-E6 embryos), and cross-species comparisons. ATAC-seq and WGBS mapped chromatin accessibility and methylation patterns, respectively. Genome editing utilized PiggyBac transposition and CRISPR/Cas9.

resultsSheep ESCs derived under TePR condition (termed TePR-sESCs) exhibited stable morphology and trilineage differentiation. Transcriptomics showed TePR-sESCs resemble 8-cell/morula embryos, consistent with sheep genome activation timing. ATAC-seq revealed accessible chromatin at pluripotency loci (e.g., POU5F1, NANOG). WGBS identified hypomethylation in pluripotency-associated regions. Efficient mCherry integration and MSTN knockouts confirmed editing compatibility.

conclusionThe TePR system enables robust derivation of sheep ESCs with embryonic-like pluripotency. TePR-sESCs' editing proficiency supports applications in livestock trait engineering and biomedical modeling, overcoming a major barrier in ungulate stem cell research.

Indexed as

Embryonic Stem CellsAgricultureAnimalsBlastocystCell Culture TechniquesCell DifferentiationCRISPR-Cas SystemsGene EditingPluripotent Stem CellsSheepEmbryonic stem cellsGenome editingMulti-omicsSheep

Identifiers

PMID40712808
PMCPMC13001039

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.