Evidence map›Paper›PMID 40715045›Full record

ArticleCell death & disease2025

The deubiquitinase USP24 suppresses ferroptosis in triple-negative breast cancer by stabilizing DHODH protein.

Li Yang, Xiaoqin An, Shangzhu Yang, Xiaowen Lin, Ziyuan Chen, Qian Xue, Xi Chen, Yuan Wang, Ding Yan, Shirui Chen and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell death & disease, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Li Yang *Department of Physiology, School of Basic Medical Sciences, Guizhou Medical University, Anshun, Guizhou, China. yangli@gmc.edu.cn.
Xiaoqin An *Department of Physiology, School of Basic Medical Sciences, Guizhou Medical University, Anshun, Guizhou, China.
Shangzhu YangDepartment of Physiology, School of Basic Medical Sciences, Guizhou Medical University, Anshun, Guizhou, China.
Xiaowen LinKey Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong, China.
Ziyuan ChenKey Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong, China.
Qian XueGuangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Disease, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, Guangdong, China.
Xi ChenGuangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Disease, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, Guangdong, China.
Yuan WangKey Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong, China.
Ding YanKey Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong, China.
Shirui ChenDepartment of Physiology, School of Basic Medical Sciences, Guizhou Medical University, Anshun, Guizhou, China.
Yuqing FanDepartment of Physiology, School of Basic Medical Sciences, Guizhou Medical University, Anshun, Guizhou, China.
Daolin TangDepartment of Surgery, UT Southwestern Medical Center, Dallas, TX, USA.ORCID http://orcid.org/0000-0002-1903-6180
Wenfeng YuDepartment of Physiology, School of Basic Medical Sciences, Guizhou Medical University, Anshun, Guizhou, China. wenfengyu@gmc.edu.cn.
Jinbao LiuGuangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Disease, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, Guangdong, China. jliu@gzhmu.edu.cn.ORCID http://orcid.org/0000-0003-2220-2407
Xin ChenKey Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong, China. chenxin@gzhmu.edu.cn.ORCID http://orcid.org/0000-0003-3668-4830

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Triple-negative breast cancer (TNBC) is an aggressive subtype of invasive breast cancer characterized by limited treatment options and a poor prognosis. While ferroptosis, an iron-dependent form of regulated cell death, plays a role in tumor suppression, its specific molecular mechanisms in TNBC remain largely unexplored. In this study, we identify deubiquitinase USP24 as the most significantly altered enzyme among key deubiquitinating enzymes during ferroptosis in human TNBC cells. Silencing USP24 enhances ferroptosis-mediated tumor suppression in TNBC cells. Mechanistically, USP24 interacts directly with dihydroorotate dehydrogenase (DHODH) and deubiquitinates it, a process critical for maintaining coenzyme Q reduction and protecting cells from lipid peroxidation. Consistently, pharmacological inhibition of USP24 synergizes strongly with ferroptosis inducers in both in vitro and in vivo models via a DHODH-dependent pathway. These findings highlight USP24 as a potential therapeutic target to enhance ferroptosis sensitivity in TNBC.

Indexed as

FerroptosisOxidoreductases Acting on CH-CH Group DonorsTriple Negative Breast NeoplasmsUbiquitin ThiolesteraseAnimalsCell Line, TumorDihydroorotate DehydrogenaseFemaleHumansMiceMice, NudeProtein StabilityDihydroorotate DehydrogenaseOxidoreductases Acting on CH-CH Group DonorsUbiquitin Thiolesterase

Identifiers

PMID40715045
PMCPMC12297596

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.