Evidence mapPaperPMID 40716099Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2025

Exosome-Associated MTA1 in Circulation Is Elevated During Breast Cancer Progression.

Kritisha Bhandari, Jeng Shi Kong, Takemi Tanaka, William C Dooley, Chao Xu, Bethany N Hannafon, Wei-Qun Ding

Abstract read
In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kritisha BhandariDepartment of Pathology, University of Oklahoma College of Medicine, Oklahoma City, Oklahoma, USA.
Jeng Shi KongDepartment of Pathology, University of Oklahoma College of Medicine, Oklahoma City, Oklahoma, USA.
Takemi TanakaDepartment of Pathology, University of Oklahoma College of Medicine, Oklahoma City, Oklahoma, USA.
William C DooleyPeggy and Charles Stephenson Cancer Center, Oklahoma City, Oklahoma, USA.
Chao XuPeggy and Charles Stephenson Cancer Center, Oklahoma City, Oklahoma, USA.
Bethany N HannafonPeggy and Charles Stephenson Cancer Center, Oklahoma City, Oklahoma, USA.
Wei-Qun DingDepartment of Pathology, University of Oklahoma College of Medicine, Oklahoma City, Oklahoma, USA.ORCID https://orcid.org/0000-0001-7358-3700

Funding

Tissue Pathology Shared ResourceP30CA225520 · NCI · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI James F Papin · 2018 to 2026
$27.1M
TUMOR RESISTANCE MECHANISMS TO ANTI-VEGF THERAPY IN PROSTATE CANCER (Sukyung Woo)P20GM103639 · NIGMS · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI HANNAFON, BETHANY · 2012 to 2022
$21.3M
Protein Production and Characterization CoreP30GM145423 · NIGMS · UNIVERSITY OF OKLAHOMA · PI ANN H WEST · 2022 to 2026
$6.9M
Axio SWhole slide scanning fluorescence and bright field microscopy with image analysis (ZEISScan.Z1)S10OD026744 · OD · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI FUNG, KAR-MING ARMIN · 2019 to 2019
$187k
Congressional-Directed Medical Research Programs W81XWH-22-1-0317HHS | NIH | National Institute of General Medical Sciences (NIGMS) 1S10OD026744HHS | NIH | National Institute of General Medical Sciences (NIGMS) P20GM103639HHS | NIH | National Institute of General Medical Sciences (NIGMS) P30GM145423NCI NIH HHS P30 CA225520NCI NIH HHS P30CA225520NIGMS NIH HHS P20 GM103639NIGMS NIH HHS P30 GM145423NIH HHS S10 OD026744Peggy and Charles Stephenson Cancer CenterPresbyterian Health Foundation (PHF)
6 · The paper itself

Abstract

Metastasis-associated protein 1 (MTA1) is overexpressed in breast cancer cells, and the MTA1 expression level is correlated with the metastasis and progression of breast cancer. We recently reported that MTA1 is transferred via exosomes from breast cancer cells to adjacent cells and to the circulation of breast cancer patients. However, whether exosome-associated MTA1 may serve as an indicator of breast cancer progression remains unknown. Wild-type MDA-MB-231 cells, MDA-MB-231 cells with MTA1 knockout, and human stromal cells were used to confirm MTA1 transfer via exosomes. Plasma was collected from nude mice (n = 6) implanted with MDA-MB-231 cells or MTA1-knockout MDA-MB-231 cells. Serum from patients with breast cancer (n = 59) and age- and sex-matched healthy subjects was collected. Exosomes from the plasma/serum were isolated via double filtration followed by the polymer precipitation method. Western blotting and ELISA were performed to detect MTA1. Knockout of MTA1 in MDA-MB-231 cells significantly reduced the tumor volume in nude mice. ELISA analysis of mouse plasma exosomes revealed that the level of exosome-associated MTA1 was significantly greater in mice implanted with wild-type MDA-MB-231 cells than in mice without implantation, and was reversed in mice implanted with MDA-MB-231 MTA1-knockout cells. The MTA1 level in serum-derived exosomes was significantly elevated in breast cancer patients compared to the matched healthy subjects. Detailed analysis revealed that exosome-associated MTA1 levels were significantly elevated in patients with stage III and IV breast cancer and in those with ER/PR-positive and Her2-positive tumors. ROC curve analysis revealed that serum exosome-associated MTA1 levels were highly accurate in predicting late-stage breast cancer.

Indexed as

Breast NeoplasmsExosomesHistone DeacetylasesRepressor ProteinsAnimalsBiomarkers, TumorCell Line, TumorDisease ProgressionFemaleHumansMiceMice, Inbred BALB CMice, NudeMiddle AgedTrans-ActivatorsBiomarkers, TumorHistone DeacetylasesMTA1 protein, humanRepressor ProteinsTrans-Activatorsbreast cancercirculating biomarkerexosomesmetastasisMTA1

Identifiers

PMID40716099
PMCPMC12704216

What Socratic holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.